HPLC法测定谷维素片中γ-谷维素的含量

Determination of γ-oryzanol in oryzanol tablets by HPLC

目的:采用HPLC法测定谷维素片中γ-谷维素的含量。方法:用基准乙醇超声对样品进行前处理,以氨基柱为分析柱,基准乙醇为流动相,流速为0.6 m L·min^(-1);检测波长为327 nm。结果:γ-谷维素平均加样回收率为100.0%,RSD=0.3(n=9),0.10~1.0μg范围内,进样量与峰面积值呈良好的线性关系(r=0.999 9,n=7)。结论:本法专属性强,操作安全简便,结果准确可靠。

Objective： To establish an HPLC method for determination of γ-oryzanol in Oryzanol Tablets. Methods： The sample was pretreated with reference ethanol by ultrasonic method. The test was performed in amino column with reference ethanol as the mobile phase. The flow rate of 0. 6 m L·min^-1,and the detection wavelength of327 nm. Results： The average spiked recovery of γ-oryzanol was 100. 0% with RSD = 0. 3（ n = 9）. There was a good linear relationship between the injection volume and peak area in the range of 0. 10-1. 0 μg,（ r = 0. 999 9,n = 7）. Conclusion： This established method is highly specific,safe and simple with good accuracy and reliability.