小鼠脂肪前体细胞成棕脂能力与增龄相关性及其机制的探究

Correlation between brown adipogenic capacity of preadipocytes and ageing and the related mechanism in mice

目的 :探讨小鼠脂肪前体细胞成棕色脂肪能力与增龄的相关性及其分子机制。方法 :体外培养不同年龄段小鼠的脂肪前体细胞,采用油红O染色鉴定前体细胞成棕脂能力;采用实时定量PCR检测年轻组和年老组小鼠脂肪前体细胞中微小RNA(micro RNA,miR)-146b-3p表达情况;通过转染miR-146b-3p模拟物或抑制物,使其过表达或抑制其表达,实时定量PCR法检测相关的脂联素、脂滴包被蛋白、脂肪酸结合蛋白4、解偶联蛋白-1基因的表达情况。结果:年老组小鼠的脂肪前体细胞成棕脂分化能力较年轻组小鼠明显减弱,同时年老组小鼠的脂肪前体细胞miR-146b-3p表达也下降。过表达miR-146b-3p可改善年老组小鼠脂肪前体细胞的成棕脂能力,而年轻组小鼠脂肪前体细胞的成脂能力随miR-146b-3p表达下降而降低。结论:脂肪前体细胞向棕色脂肪细胞分化的能力随着年龄的增加而降低,miR-146b-3p可能是其中的一个作用靶点。

Objective： To investigate correlation between brown adipogeneic capacity of preadipocytes and ageing and the related mechanism in mice. Methods： Preadipocytes derived from various age mice were cultured in vitro, and oil red O staining was used to identify the brown adipogenic capacity. The expression of miR-146b-3p in young and old preadipocytes was detected by real-time quantitative PCR. Then miR-146b-3p inhibitors were transfected into young preadipocytes, while miR-146b-3p mimics were transfected into the old, and the relative mRNA levels of PLIN, FABP4, UCP-1, A PN were measured by real-time quantitative PCR. Results： Preadipocytes of aged mice showed a weaker capacity of brown adipogenic capacity than that of the young mice, and the expression of miR-146b-3p in the preadipocytes of the aged group was also decreased. Overexpression of miR-146b-3p can improve the brown adipogenic capacity of preadipocytes in aged mice. However, the brown adipogenic capacity of the young declined with the decrease of miR- 146b-3p expression. Conclusions： The brown adipogenic capacity of preadipocytes decreases with age, and miR-146b-3p might be one of the targets.