120例不孕不育患者染色体核型分析及与临床生殖异常的相关性研究

Analysis of karyotype analysis of 120 infertile patients and correlation with clinical reproductive abnormality

目的探讨不孕不育患者染色体核型及与临床生殖异常的相关性。方法选择2015年8月-2017年8月入院治疗的不孕不育患者120例,设为观察组;选择同期入院正常健康体检者120例,设为对照组。观察组所有患者完成染色体核型分析;记录并统计120例患者临床生殖异常情况;采用SPSS Pearson相关性分析软件对不孕不育患者染色体核型与临床生殖异常相关性进行分析。结果 120例不孕不育患者均完成染色体核型分析,47,XXY、47,XYY均为男性表型,临床多表现为睾丸发育不全,精子数量或存活率低;45,XO为女性表型,临床表现为发育不全;120例不孕不育患者中28例染色体结构或功能异常,占23.33%。排在前两位的分别为46,XY,t(9;Y)(q32;q12)临床表现为配偶未孕,精子成活率较低或数量少、46,XY,del(X)(q21)临床表现为外阴发育不良,未孕;观察组与对照组胎儿畸形率比较差异无统计学意义(P>0.05);观察组复发性流产、自然流产及不孕不育发生率,均低于对照组(P<0.05);SPSS Pearson相关性分析结果表明:不孕不育患者生殖异常与染色体数量、染色结构或功能异常呈正相关性(P<0.05)。结论不孕不育患者多伴有染色体核型数量、结构或功能异常,是造成临床生殖异常的重要原因,加强染色体核型测定能了解患者不孕不育原因,为临床治疗提供依据和参考,值得推广应用。

Objective：To explore the relationship between karyotype and infertility in clinical infertility patients. Methods：A total of 120 infertile patients admitted to hospital from August 2015 to August 2017 were selected as observation group;120 patients were selected as the normal control group. All patients in the observation group completed chromosome karyotype analysis;120 cases were recorded and counted for clinical reproductive abnormalities;SPSS Pearson correlation analysis software was used to analyze the correlation between karyotype and clinical reproductive abnormalities in infertility patients. Results：The karyotype analysis was completed in 120 cases of infertility. 47,XXY,47,XYY were all male phenotypes. Most clinical manifestations were testicular hypoplasia,low sperm count or survival rate;45,XO was female Type,clinical manifestations of hypoplasia;120 cases of infertility in 28 patients with abnormal chromosome structure or function,accounting for 23.33%. The first two were 46,XY,t（9;Y）（q32;q12）. The clinical presentation was that the spouse was not pregnant,and the sperm survival rate was low or low,46,XY,del（X）（q21）The clinical manifestations were hypoplasia of the vulva and non-pregnancy;there was no significant difference in the fetal malformation rate between the observation group and the control group（P〉0.05）;the incidence of recurrent miscarriage,spontaneous abortion and infertility was lower in the observation group than in the control group. Group（P〈0.05）;SPSS Pearson correlation analysis showed that：reproductive infertility patients were positively correlated with chromosome number,staining structure or function abnormalities（P〈0.05）. Conclusion：Infertility patients are often associated with abnormal numbers,structure,or function of karyotypes,which is an important cause of clinical reproductive abnormalities. Strengthening chromosome karyotype determination can understand the causes of infertility in patients and provide a basis and reference for clinical treatment. It is worth promoting.