+G_Z重复暴露对大鼠脑组织c-fos、c-jun和神经生长因子基因表达的影响

Altered mRNA Expression of C fos, C jun and Nerve Growth Factor Gene in the Brain of Rat Exposured to Repeated +G_Z

目的：为了解＋ ＧＺ 重复暴露后大鼠脑组织ｃｆｏｓ、ｃｊｕｎ 和神经生长因子（ＮＧＦ） 基因表达的变化，探讨它们在＋ ＧＺ 所致脑损伤中的作用和意义。方法：用半定量反转录聚合酶链反应（ＲＴＰＣＲ） 检测方法检测＋ ＧＺ 重复暴露后大鼠脑组织ｃｆｏｓ、ｃｊｕｎ 和ＮＧＦ ｍ ＲＮＡ 表达水平。结果：ｃｆｏｓ 和ｃｊｕｎ 在＋ ＧＺ 重复暴露后３０ ｍｉｎ ｍ ＲＮＡ 表达明显升高，６ｈ 和２４ｈ 降至正常；ＮＧＦ 在３０ ｍｉｎ 和６ ｈ 表达均明显升高，２４ ｈ 恢复正常。结论：表明＋ ＧＺ 重复暴露可诱导大鼠脑组织ｃｆｏｓ、ｃｊｕｎ 和ＮＧＦ ｍ ＲＮＡ 的表达，提示它们的表达增加可能在＋ ＧＺ 所致脑损伤中起病理或保护作用。

To study the changes of mRNA expression of c fos, c jun and nerve growth factor (NGF) in the brain of rat exposured to repeated +G Z, and to explore the role of them in brain damage induced by repeated +G Z exposures, the mRNA expression levels of c fos, c jun and NGF in rat brain exposured to repeated +G Z were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR). The results showed that the c fos and c jun mRNA expression levels in rat brains taken 30 min, NGF mRNA expression levels in rat brains taken 30 min and 6 h after repeated +G Z exposures were significantly higher than those of control group. It is suggested that c fos, c jun and NGF mRNA expression in rat brain can be inducted by repeated +G Z exposures and they may poly an important role in the pathologic course or self protection effection of brain neurons damage induced by+G Z exposures.