正常豚鼠ACS-VEMPs动物检测方法的建立

Vestibular-evoked myogenic potentials in healthy guinea pigs

目的:建立动物实验VEMPs检测方法,确定正常豚鼠气导声刺激(ACS)的颈肌前庭诱发肌源性电位(cVEMP)、眼肌前庭诱发肌源性电位(oVEMP)的实验方法和波形引出特点,从而为cVEMP和oVEMP的后续研究奠定实验基础。方法:选择11只健康豚鼠(250～350g),使用特制的动物固定架,保持动物俯卧抬头位,在清醒状态下行ACS-cVEMP、ACS-oVEMP,建立正常豚鼠的VEMPs测试方法并记录分析波形特点和参数结果。结果:11只(22耳)健康豚鼠均可引出气导声刺激诱发的ACS-cVEMP和ACS-oVEMP。ACS-cVEMP和ACS-oVEMP的引出率分别为77.27%和59.09%;阈值分别为(107.1±14.6)dB SPL和(115.5±15.6)dB SPL;cVEMP的n1、p1潜伏期分别为(7.4±1.5)ms和(9.3±1.6)ms,oVEMP的n1、p1潜伏期分别为(6.4±1.4)ms和(8.1±1.8)ms;振幅cVEMP和oVEMP分别为(15.4±8.6)μV和(11.4±6.0)μV。在阈值、n1潜伏期、p1潜伏期和振幅参数方面,cVEMP和oVEMP无统计学差异(P>0.05)。结论:通过本实验方法,成功在动物(豚鼠)身上诱发出稳定的气导声刺激诱发的VEMPs波形,包括cVEMP和oVEMP,并测得正常豚鼠VEMPs的参数值,包括引出率、阈值、潜伏期和振幅,为将来各种内耳疾病评估前庭功能的模型奠定了良好的实验基础。

Objective：To establish detection methods of air-conducted sound elicited vestibular-evoked myogenic potentials（ACS-VEMPs）in guinea pigs,including cervical vstibular-evoked myogenic potential（cVEMP）and ocular vestibular-evoked myogenic potential（oVEMP）.Method：Eleven healthy（250-350 g）guinea pigs（22 ears）were selected and underwent conventional ACS-cVEMP and ACS-oVEMP examinations.Parameters of waveforms were recorded.Result：The ACS-cVEMP and ACS-oVEMP could be elicited in 77.27% and 59.09% guinea pigs,respectively;threshold was（107.1±14.6）dB SPL and（115.5±15.6）dB SPL for ACS-cVEMP and ACS-oVEMP;n1,p1 latency for cVEMP was（7.4±1.5）ms and（9.3±1.6）ms,（6.4±1.4）ms and（8.1±1.8）ms for oVEMP;amlipude was（15.4±8.6）μV and（11.4±6.0）μV for cVEMP and oVEMP respectively.No statistical difference could be found in above parameters between ACS-cVEMP and ACS-oVEMP（P0.05）.Conclusion：Both ACS-cVEMP and ACS-oVEMP could be elicited in guinea pigs.