蒜芥茄SsUBC基因的克隆及表达分析

Cloning and Expression Analysis of SsUBC Gene in Solanum sisymbriifolium Lam.

本研究从蒜芥茄中克隆得到泛素E2结合酶UBC基因编码区序列,命名为SsUBC,登陆Gen Bank,编号:MF872897。SsUBC基因编码区全长859 bp,编码272个氨基酸。进行进化树分析,SsUBC编码的氨基酸序列与番茄和马铃薯等茄科植物有较近的亲缘性。利用荧光定量PCR技术分析SsUBC基因在蒜芥茄各器官表达特征和不同非生物胁迫处理下(镉,高温,干旱,盐和脱落酸)根部和叶片的表达特性。结果表明,SsUBC基因表达在不同器官中具有差异性,且叶片中表达量最高。非生物胁迫处理后叶片中SsUBC基因表达变化比根部中更为显著,除盐胁迫外,其它四种处理条件下该基因在叶片中的表达量均高于根部,其中高温胁迫下表达量升高变化最为显著。除盐胁迫外,叶片中基因的表达均为上调趋势,而干旱胁迫下根中该基因表达为下调趋势。综上,SsUBC基因在蒜芥茄响应镉(1 h)、高温(1 h)、干旱胁迫(1 h)时的应答较为迅速,其次为脱落酸(9 h),且响应部位为叶片;而在盐胁迫(12 h)下应答相对较为迟缓。

In this study,we cloned the ubiq uitin E2 binding enzyme UBC gene coding region sequence from Solanum sisymbriifolium,which was named as SsUBC,and its accession number in Gen Bank was No.MF872897.The coding region of SsUBC gene was 859 bp in length,encoding 272 amino acids.Phylogenetic tree analysis showed that the amino acid sequence encoded by SsUBC was closely related to solanaceous plants,such as tomato and potato.The expression characteristics of SsUBC gene in different organs of Solanum sisymbriifolium and in roots and leaves under different abiotic stress（cadmium,high temperature,drought,salt and abscisic acid） were studied by fluorescence quantitative PCR.The results indicated that the expression of SsUBC gene was different in different tissues,with the highest expression in leaves.The expression changes of SsUBC gene in the leaves were more obvious than those in the roots after abiotic stress.In addition to salt stress,the expression of SsUBC gene in leaves was higher than that in roots under the other four treatments.The expression level of SsUBC gene increased significantly under high temperature stress.The most significant change.The expression of the genes in the leaves increased except after salt stress,while the expression of the genes in the roots under drought stress decreased.In summary,the response of SsUBC gene was rapid in response to cadmium（1 h）,high temperature（1 h） and drought stress（1 h）,followed by abscisic acid（9 h）,and the response site was leaf.However,under salt stress（12 h）,the response was relatively slow.