电针对肥胖大鼠白色脂肪组织过氧化物酶体增殖物激活受体γ辅激活因子1α、解耦联蛋白1的影响

Electroacupuncture Relieves Obesity by Up-regulating PGC-1 α/UCP-1 Signaling in White Adipose Tissue in Diet-induced Obesity Rats

目的:研究电针对高脂饮食诱导的肥胖(DIO)大鼠白色脂肪组织(WAT)中过氧化物酶体增殖物激活受体γ辅激活因子1α/解耦联蛋白1(PGC-1α/UCP-1)信号通路的影响,探讨针灸减肥的作用机制。方法:Wistar雄性大鼠随机分为对照组(10只)和造模组(40只),高脂饲料喂养建立营养型肥胖动物模型。造模成功的大鼠随机分为模型组、穴位组和非穴位组(8只/组)。穴位组取"足三里""天枢"穴,非穴位组取"足三里""天枢"穴旁开5 mm区域的非穴位区进行电针,每次30min,每周5次,共治疗8周。每周测量1次各组大鼠体质量、体长,并计算Lee’s指数;采用生化法检测大鼠的血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)水平;免疫组化法检测大鼠WAT中PGC-1α、UCP-1蛋白表达。结果:治疗后,与对照组比较,模型组大鼠的Lee’s指数、TC、TG水平均显著升高(P<0.05),HDL-C水平及PGC-1α、UCP-1蛋白表达显著降低(P<0.05)。与模型组和非穴位组比较,穴位组大鼠的Lee’s指数、TC、TG水平均显著降低(P<0.05),HDL-C水平及PGC-1α、UCP-1蛋白表达显著升高(P<0.05)。结论:电针可以有效调控WAT中PGC-1α、UCP-1的表达,这可能是电针通过促进WAT"棕色化"达到减肥效应的机制之一。

Objective To observe the effect of electroacupuncture（EA）on the activities of peroxisome proliferatoractivated receptor gamma coactivator-1 alpha/uncoupling protein-1（PGC-1α/UCP-1）signaling pathway in white adipose tissue（WAT）of diet-induced obesity（DIO）rats,so as to reveal its underlying mechanism in body weight loss.Methods Thirty-four male Wistar rats were randomly divided into normal diet（control,n=10）,high fat diet（model）,sham EA-acupoint and EA groups（n=8 in each of the latter 3 groups）.The obesity model was established by feeding the rats with high fat diet containing lard oil,sugar,albumen powder,cholesterol,salt and sodium cholate for 12 weeks.EA（2 Hz/15 Hz,1 mA）was applied to bilateral＂Zusanli＂（ST 36）and ＂Tianshu＂（ST 25）or sham acupoints（about 5 mm beside ST 36 and ST 25）for 30 min,once daily,5 times per week for a total of 8 weeks.During the treatment,all rats were fed with normal diet,and their body weight and length were measured once a week for calculating the Lee＇s index.The contents of serum total cholesterol（TC）and triglyceride（TG）were measured by using biochemical methods.The immunoactivity of PGC-1αand UCP-1 in the abdominal WAT was detected by immunohistochemistry.Results After modeling,the Lee＇s index,serum TC and TG contents were significantly increased,and the levels of serum HDL-C,and PGC-1αand UCP-1 immunoactivity in WAT considerably decreased in the model group relevant to the control group（P〈0.05）.Following the treatment,the Lee＇s index,TC and TG contents were significantly down-regulated while HDL-C and PGC-1αand UCP-1 immunoactivity were obviously up-regulated in the EA-acupoint group relevant to the model group（P〈0.05）.Conclusion EA can effectively reduce the body weight and adipose content in obesity rats,which may be closely related to its effect in up-regulating PGC-1α/UCP-1 signaling in WAT,suggesting an efficacy of EA in promoting the browning of WAT.