火针对胶原诱导型关节炎大鼠血清中肿瘤坏死因子-α和抗环瓜氨酸多肽抗体含量的影响

Fire-needle Acupuncture Intervention Relieves Ankle-joint Inflammatory Reactions Possibly by Down-regulating Serum TNF-α and Anti-cyclic Citrullinated Peptide Antibody Levels in Collagen-induced Arthritis Rats

目的:观察火针对胶原诱导型关节炎(CIA)大鼠血清肿瘤坏死因子-α(TNF-α)和抗环瓜氨酸多肽抗体(ACPA)含量的影响,探讨火针对类风湿关节炎的作用机制。方法:Wistar大鼠随机分为空白组、模型组、甲氨蝶呤组和火针组,每组8只。采用鸡Ⅱ型胶原注射大鼠右足底部、背部和尾根部制备CIA模型。甲氨蝶呤组予0.1mg/100g的甲氨蝶呤氯化钠溶液灌胃,1次/5d,共2次;火针组予火针速刺"足三里""昆仑",1次/d,左右腿交替进行,共10次。监测各组大鼠的右踝关节直径和体质量。干预结束后拍摄大鼠右踝X线片,ELISA法检测大鼠血清TNF-α和ACPA含量。结果:X线片可见模型组右足跗部关节肿胀变形,关节间隙狭窄,甲氨蝶呤组和火针组较模型组改善,关节肿胀变轻,骨骼畸形较轻。干预后,与空白组比较,模型组右踝直径及血清TNF-α、ACPA含量升高(P<0.05);与模型组比较,甲氨蝶呤组和火针组右踝直径及血清TNF-α、ACPA含量下降(P<0.05);甲氨蝶呤组和火针组各指标比较差异无统计学意义(P>0.05)。结论:火针对CIA大鼠有类似甲氨蝶呤的治疗作用,其机制之一可能是火针可降低血清TNF-α和ACPA的含量。

Objective To observe the effect of fire-needle acupuncture of＂Zusanli＂（ST 36） and ＂Kunlun＂（BL 60）on ankle-joint swelling and serum tumor necrosis factor-α（TNF-α）and anti-cyclic citrullinated peptide antibody（ACPA）contents in collagen-induced arthritis（CIA）rats,so as to study its mechanism underlying improvement of rheumatoid arthritis（RA）.Methods Thirty-two male Wistar rats were randomized into control,model,medication（Methotrexate）and fire-needling groups（n=8 in each）.The RA model was established by injecting typeⅡchicken collagen（0.1 mol/L）plus Freund＇s complete adjuvant（primary immunization）and Freund＇s incomplete adjuvant（immunization once more）into the subcutaneous tissues of the right foot bottom,back and tail root of rats.Fire-needling was applied to the left and right ＂Zusanli＂（ST 36） and ＂Kunlun＂ （BL 60）alternatively for3 times in each acupoint,once daily for 10 days.For rats of the medication group,Methotrexate sodium chloride solution（0.1 mg/100 g）was administrated by gavage,once every 5 days,twice together.The rats＇ right hind ankle diameter was measured before and after the treatment.The X-ray film of the right ankle was taken,and the contents of serum TNF-αand ACPA were assayed by ELISA.Results The ankle diameter and serum concentrations of TNF-αand ACPA were significantly increased in the model group compared to the control group（P〈0.05）,and X-ray film showed swollen and deformed tarsus joints,and narrowing of the joint space.After the intervention,the ankle diameter,serum TNF-αand ACPA levels were considerably decreased in both medication and fire-needle groups compared with the model group（P〈0.05）.Meanwhile,the joint swelling and bone deformity became relatively milder.There were no significant differences between the medication and fire-needling groups in the ankle diameter and the contents of serum TNF-αand ACPA（P〉0.05）.Conclusion Fire-needling stimulation of ST 36 and BL 60,similar to Methotrexate,can relieve the inflammatory reactions of hind-ankle joint in CIA rats,which may be related to its effect in down-regulating ACPA and TNF-αlevels.