LED光质对乌饭树组培苗茎段增殖和生根的影响

Effect of Different LEDs Light Qualities on Vaccinium bracteatum Stem Multiplication and Rooting

采用发光二极管(LED)调制光质,以荧光灯为对照,研究LED不同红蓝光质比对乌饭树茎段增殖及生根的影响,为利用光质调控技术提高乌饭树组培效率和品质提供科学依据。结果表明,红光有利于新梢伸长及叶面积增加,但显著抑制叶绿素合成;蓝光抑制茎段新梢的诱导,但能促进组培苗不定根形成。红蓝复合光最有利于乌饭树组培苗茎段增殖、生物量积累以及根系发育,其中,在70%R(红光)+30%B(蓝光)处理下,带芽茎段的新梢诱导率、新梢数、叶片数、鲜重和干重均达到最大;而在生根阶段,组培苗的生根率、根数和根长在50%R+50%B处理下达到最大。此外,在单色蓝光处理下叶绿素a/b比值最大,是红光处理下的1. 46倍;红蓝复合光促进乌饭树组培苗类胡萝卜素的合成。与荧光灯相比,适宜的LED光质能显著促进乌饭树茎段增殖和生根,是替代荧光灯的理想光源。

We discussed the effect of light-emitting diodes（LEDs） light qualities on the multiplication and rooting of Vaccinium bracteatum . The application of light quality control will offer scientific references to improve the efficiency and quality of V.bracteatum tissue culture. The light quality change was controlled by the LEDs, and the fluorescent was set as control. Different rations of red and blue lights set were listed below： 100% red, 70% red＋30% blue, 50% red＋50% blue, 30% red＋70% blue and 100% blue. The study released that the red light was benefit for new shoot multiplication and leaf area increase, and limited for chlorophyll synthesis significantly; and blue light was benefit for adventitious roots form and limited for shoot formation. The complex of red and blue lights was best for stem growth, biomass accumulation, and root development in V.bracteatum tissue culture. In addition, with the light quality of 70% red＋30% blue, the shoot induction rate, number of shoots, number of leaves, fresh and dry weights of the shoots with buds reached the maximum values. During the rooting stage, the light quality of 50% red＋50% blue was best for rooting rate, root number and root length in culture tissues. The ratio of chlorophyll a/b got the highest value at pure blue light condition, and the number is 1.46 times than that at red light condition. The complex lights could increase carotenoid concentrations in V.bracteatum tissues. Therefore, LEDs could improve V.bracteatum shoot multiplication and rooting, and LEDs could be an optimization than the fluorescent.