ICP-MS及AAS法测定川明参的无机营养元素

Assay of Inorganic Nutrient Elements in Chuanmingshen violaceum Roots by ICP-MS and AAS

目的:建立川明参药材中无机营养元素含量的测定方法,并比较不同产地川明参的营养元素。方法:从四川省8个川明参种植县/区采集23份川明参样品,根据地理位置,将其产地分为A(德阳市中江县和成都市金堂县和青白江区)、B(绵阳市盐亭县)、C(南充市阆中市、巴中市巴州区、广元市苍溪县)、D(达州万源市)产区。采用微波消解-ICP-MS及AAS法测定这些样品中13种营养元素的含量,并运用主成分分析法及t检验法对其所含元素进行评价。结果:23份川明参样品中13种无机营养元素的含量均在一定范围之内,平均值分别为0.026μg/g(Li)、6.105μg/g(B)、7.638μg/g(Mn)、56.139μg/g(Fe)、0.031μg/g(Co)、3.695μg/g(Zn)、0.041μg/g(Se)、7.252μg/g(Sr)、0.022μg/g(Mo)、115.417μg/g(Na)、785.896μg/g(Mg)、7 356.074μg/g(K)、282.205μg/g(Ca)。尽管这些不同产地样品中元素的含量不同,但各自的指纹图谱却呈相似的波浪式分布态势。t检验结果表明,Se、Zn、Mn、Co、Fe、Mg元素的含量在4个产区之间有显著性差异,其余元素含量差异不显著。主成分分析法提取前6个因子的综合得分模型为F=3.295C_1+2.341C_2+2.131C_3+1.362C_4+1.061C_5+0.734C_6。4个产区综合得分由高至低的顺序为D(0.917,n=5)、A(0.180,n=6)、B(-0.381,n=3)、C(-0.400,n=9)。结论:ICP-MS法和AAS法能够快速、准确的测定川明参中营养元素的含量。以无机营养元素为指标,川明参的营养价值较高,为滋补佳品。从无机营养元素的含量考虑,D产区(达州万源市)为栽培川明参的适宜产区。

Objective： To develop a method for determination of inorganic elements in Chuanmingshen violaceum,and to evaluate the nutrient value of herbal samples among different cultivation areas. Methods： A total of 23 Chuanmingshen violaceum samples were collected from eight counties in Sichuan. The cultivation area of these samples were assigned as four producing areas,i. e. A（ Zhongjiang County,Jintang County and Qingbaijiang District）,B（ Yanting County）,C（ Langzhong City,Bazhou District and Cangxi County） and D（ Wanyuan City） based on the geographical sites and ecological condition. The 13 inorganic nutrient elements were quantified in these Chuanmingshen violaceum samples by inductively coupled plasma-mass spectrometry（ ICP-MS） and atomic absorption spectrophotometry（ AAS）. The amounts of inorganic elements and nutrient value were compared and evaluated among these herbal samples and producing areas by principal component analysis（ PCA） and t-test. Results： The contents of the 13 inorganic elements in the 23 herbal samples were within a certain range. The mean were 0. 026 μg/g（ Li）,6. 105 μg/g（ B）,7. 638 μg/g（ Mn）,56. 139 μg/g（ Fe）,0. 031 μg/g（ Co）,3. 695 μg/g（ Zn）,0. 041 μg/g（ Se）,7. 252 μg/g（ Sr）,0. 022 μg/g（ Mo）,115. 417 μg/g（ Na）,785. 896 μg/g（ Mg）,7356. 074 μg/g（ K） and 282. 205 μg/g（ Ca）. Although the contents of these elements in different Chuanmingshen violaceum samples were not consistent,their fingerprints were similar in a regular wave profile,which was a characteristic for its genuine identification. The results of t-test analysis indicated that Se,Zn,Mn,Co,Fe,Mg contents were significantly different（ P 0. 05） among the four producing areas. The differences of amounts for other elements were not significant（ P0. 05）. Six factors were extracted and used to comprehensively evaluate the nutrient value for a specific Chuanmingshen violaceum sample by PCA analysis. The comprehensive function was F =3. 295 C1＋2. 341 C2＋2. 131 C3＋1. 362 C4＋1. 061 C5＋0. 734 C6. The order of score among the four producing areas were 0. 917（ n = 5,D）,0. 180（ n = 6,A）,-0. 381（ n = 3,B） and-0. 400（ n = 9,C）. Conclusion： The developed ICP-MS and AAS method is a rapid andaccurate method for the determination of inorganic elements in Chuanmingshen violaceum. Chuanmingshen violaceum is good nutrient containing rich inorganic nutrient elements. Wanyuan City in Sichuan of China is the suitable and good cultivation area for Chuanmingshen violaceum.