金佛山雪胆块根的化学成分研究

Chemical Constituents from the Tubers of Hemsleya pengxianensis var. jinfushanensis

目的:研究金佛山雪胆块根乙酸乙酯部位的化学成分。方法:利用硅胶柱色谱、ODS反相柱色谱、HPLC制备色谱等技术分离纯化化合物,根据化合物的波谱数据分析鉴定化合物结构。结果:从金佛山雪胆块根乙酸乙酯部位中共分离鉴定了11个化合物,分别为:scandenoside R8(1)、3-O-β-D-glucopyranosyl-3β,27-dihydroxycucurbita-5,24(Z)-diene-11-one-27-O-β-D-glucopyranoside(2)、短柄雪胆苷A(3)、scandenoside R3(4)、scandenoside R4(5)、jinfushanoside J(6)、carnosiflosideⅤ(7)、carnosiflosideⅢ(8)、scandenoside R6(9)、jinfushanoside K(10)、雪胆双糖(11)。结论:其中,化合物4、5、7、9、11为首次从该植物中分离得到,并首次归属了化合物11的羟基信号。

Objective： To study the chemical constituents of the ethyl acetate extract of the tubers of Hemsleya pengxianensis var. jinfushanensis. Methods： The compounds were isolated by various chromatographic techniques,including silica gel,ODS reversed phase column and preparative high-performance liquid. The structures were identified on the basis of MS and NMR data. Results： Eleven compounds were obtained from the ethyl acetate fraction of the tubers of Hemsleya pengxianensis var. jinfushanensis and identified as scandenoside R8（ 1）,3-O-β-D-glucopyranosyl-3β,27-dihydroxycucurbita-5,24（ Z）-diene-11-one-27-O-β-D-glucopyranoside（ 2）,delavanoside A（ 3）,scandenoside R3（ 4）,scandenoside R4（ 5）,jinfushanoside J（ 6）,carnosifloside Ⅴ（ 7）,carnosifloside Ⅲ（ 8）,scandenoside R6（ 9）,jinfushanoside K（ 10）,amabiose（ 11）. Conclusion： Compounds 4,5,7,9,11 are isolated from this plant for the first time,and the hydroxyl signals of compound 11 are identified for the first time.