蜂海绵共附生真菌Aspergillus sp. LS06次级代谢产物研究

Study on the Secondary Metabolites from a Fungus Aspergillus sp. LS06 Associated with Haliclona sp.

目的:研究蜂海绵共附生真菌Aspergillus sp.LS06的次级代谢产物。方法:采用减压硅胶柱层析、反相中压柱层析、制备高效液相色谱等方法对Aspergillus sp.LS06的发酵产物进行分离纯化。通过理化性质及波谱分析的方法,并结合文献资料进行结构鉴定。结果:从蜂海绵共附生真菌Aspergillus sp.LS06的发酵提取物中分离鉴定了11个化合物,分别为:环(6R-羟基-L-脯氨酸-L-亮氨酸)(1)、环(6R-羟基-L-脯氨酸-L-苯丙氨酸)(2)、环(L-脯氨酸-L-亮氨酸)(3)、环(L-脯氨酸-L-酪氨酸)(4)、环(L-苯丙氨酸-甘氨酸)(5)、乙基2-(4-羟基苯基)醋酸(6)、甲基2-(2,5-二羟苯基)醋酸(7)、2-(4-甲氧基苯基)乙酸(8)、酪醇(9)、亚油酸(10)、5-羟甲基糠酸(11)。结论:所有化合物均首次从该真菌分离得到。

Objective ：To investigate the chemical constituents on a fungus Aspergillus sp. LS06 associated with the marine sponge Haliclona sp.. Methods：Secondary metabolites of AspergiUus sp. LS06 were separated employing vacuum silica gel column chromatogra- phy, medium pressure column chromatography, and high performance liquid chromatography. Their chemical structures were character- ized by the comparison of spectroscopic data and the references. Results ： Eleven compounds were isolated from the fermentation broth of AspergiUus sp. LS06, whose structures were established as cyclo-（6R-OH-L-Pro-L-Leu） （1）, cyclo-（ 6R-OH-L-Pro-L-Phe ）（2）, cyclo- （L-Pro-L-Leu） （ 3 ）, cyclo-（L-Pro-L-Tyr） （ 4 ）, cyclo-（ L-Phe-Gly ） （ 5 ）, ethyl 2-（ 4-hydroxyphenyl ） acetate （ 6 ）, methyl 2-（ 2,5-di- hydroxyphenyl） acetate （ 7 ）, 2- （ 4-methoxyphenyl ） acetic acid （ 8 ）, tyrosol （ 9 ）, linoleic acid （ 10 ） and 5-hydroxymethylfuroic acid （ 11 ）, respectively. Conclusion：All the compounds are separated from this fungus for the first time.