丹参酮Ⅱ_A下调XIAP及GRP78促进Eca109细胞凋亡

Apoptotic Effect of Tanshinone Ⅱ_A in Esophageal Squamous Eca109 Cells via Suppression of XIAP and GRP78

目的:探讨丹参酮Ⅱ_A促食管癌细胞系Eca109细胞凋亡的可能机制。方法:Eca109细胞应用不同浓度(2、4、8μg/mL)丹参酮Ⅱ_A处理48 h后,应用荧光定量实时PCR及Western blotting分析凋亡相关蛋白BAX、Bcl-2、Caspase-3、Caspase-9、GRP78、XIAP mRNA及蛋白的表达。结果:丹参酮Ⅱ_A可促进Eca109细胞凋亡,抑制Bcl-2、XIAP、GRP78 mRNA及蛋白表达,促进BAX、Caspase-9、Caspase-3 mRNA及蛋白表达,其作用呈浓度依赖性。结论:丹参酮Ⅱ_A可能通过调节凋亡相关蛋白表达促进Eca109细胞凋亡。

Objective： To investage the possible mechanisms of Tanshinone ⅡA promoting esophageal squamous cells（ Eca109 cells） apoptosis.Methods： The human esophageal squamous cells（ Eca109 cells） were treated with Tanshinone ⅡA for 48 h at different concentrations（ 2,4,8 g/mL）. The expression of apoptosis related proteins BAX,Bcl-2,Caspase-3,Caspase-9,GRP78,XIAP mRNA and protein expression were analyzed by real-time quantitative PCR（ q PCR） and Western blotting. Results： Tanshinone ⅡA inhibited the cell proliferation and induced apoptosis in Eca109 cells. The mRNA and protein expression of Bcl-2,XIAP,GRP78 were inhibited,the mRNA and protein expression of BAX,Caspase-9 and Caspase-3 were increased,which in a concentration dependent manner. Conclusion： Tanshinone ⅡA inhibits the growth of esophageal squanous cells Eca109 and induces apoptosis possibly by suppressing cell apoptosis-related XIAP and GRP78 protein expression.