遵义地产药材白及与知母HPLC指纹图谱识别

HPLC Fingerprints Identification of Bletilla striata (Thunb.) Reichb. f. and Anemarrhena Asphodeloides Bge. in ZunYi

目的:研究并建立遵义白及药材HPLC指纹图谱识别方法,方法:采用HPLC色谱法,建立指纹图谱识别共有模式。结果:建立白及药材HPLC指纹图谱识别方法,并对不同产地的白及和白及对照药材进行相似度比较,HPLC指纹图谱标定为13个共有峰,10批不同产地白及药材相似度均大于0.95,3批知母药材相似度均小于0.20。结论:白及药材HPLC指纹图谱具有重复性好、特征性强、方法简便等特点,可用于白及的有效鉴别。

Objective The HPLC fingerpint for the identification of Bletilla striata（ Thunb.） Reichb. f. Methods The mutual mode to HPLC fingerprints was set up. Results HPLC fingerprints was set up,and the 13 common peaks were obtaingd. The similarities adulterants samples were all above 0. 95,adulterant samples were less than 0. 20. Conclusion The method is simple,stable,and reproducible,which can be used to identify Bletilla striata（ Thunb.） Reichb. f.