肝内胆管细胞癌中Ezrin和PTEN蛋白的表达及其临床意义

The expressions and clinical significance of Ezrin and PTEN protein in intrahepatic cholangiocarcinoma

目的探讨肝内胆管细胞癌（ICC）中埃兹（Ezrin）蛋白和磷酸酶基因（PTEN）蛋白的表达特点及其临床意义。方法采用免疫组化sP法检测50例肝内胆管细胞癌（ICC组）和相应癌旁组织（对照组）中Ezrin和PTEN蛋白的表达情况，分析上述指标与临床病理因素的关系。结果ICC组Ezrin阳性表达率为78．0％（39／50），癌旁组织阳性表达率为46．0％（23／50）；二者差异具有统计学意义（P〈0．05）。Ezrin蛋白的表达与ICC肿瘤直径、分化程度、TNM分期、淋巴结转移相关（P〈0．05），与患者年龄、性别、CA19-9、HBsAg、肝内胆管结石无关（P〉0．05）。PTEN在ICC组织的阳性表达率为46．0％（23／50），在癌旁组织的阳性表达率为88．0％（44／50），二者差异具有统计学意义（P〈0．05）。PTEN蛋白的表达与ICC的分化程度、TNM分期、淋巴结转移相关（P〈0．05），与患者年龄、性别、CA19-9、HBsAg、肿瘤直径、肝内胆管结石无关（P〉0．05）。Ezrin和PTEN蛋白在ICC组织中的表达呈负相关（r=-0．382，P〈0．01）。结论Ezrin和PTEN在ICC中的异常表达及二者表达的负相关可能在ICC的侵袭及转移过程中起到重要作用。

Objective To study the expressions and the clinicopathologic significance of Ezrin and PTEN protein in intrahepatic cholangiocarcinoma. Methods The ICC tissues （the ICC group） and the cor- responding para-carcinoma tissues （the control group） were collected from 50 patients with intrahepatic cholangiocarcinoma （IHC） to detect the expressions of Ezrin and PTEN protein by using SP immunohisto- chemistry （IHC）. The clinicopathologic parameters were analyzed. Results The positive expression rates of Ezrin were 78.0% （39/50） and 46.0% （23/50） in the ICC group and the control group, respectively. The difference in expression between the two groups was statistically significance （P〈0.05）. The expression of Ezrin in the ICC group was highly related to tumor size, differentiation grade, TNM stage, and lymphatic metastasis （P〈0.05）, but it was not related to age, gender, serum CA19-9 level, hepatitis B virus infec- tion, intrahepatic duct calculus （P〉0.05）. The positive expression rates of PTEN was 46.0% （23/50） and 88.0% （44/50） in the ICC group and the control group, respectively. The difference in expression between the two groups was statistically significance （P〈0.05）. The expression of PTEN in the ICC group was highly related to differentiation grade, TNM stage, and lymphatic metastasis （P〈0.05）, but it was not related to age, gender, serum CA19-9 level, hepatitis B virus infection, tumor size, intrahepatic duct calculus （P〉0. 05）. There was a negative correlation between the expression of Ezrin and PTEN protein in ICC （r= -0.382, P〈0.01）. Conclusion The abnormal and negative correlation between the expression of Ezrin and PTEN protein in ICC may play an important role in invasion and metastasis of ICC.