蛛网膜下腔出血后脑心综合征大鼠体内肾素-血管紧张素的变化

Changes of renin-angiotensin in rats with cerebral-cardiac syndrome after subarachnoid hemorrhage

目的探讨蛛网膜下腔出血(SAH)并发脑心综合征(CCS)大鼠肾素-血管紧张素的变化。方法枕大池注入自体动脉血法复制SAH模型,健康雄性Wistar大鼠75只,6周龄,体重150~200 g,所有大鼠均与动力实验室系统连接(Power Lab系统)检测心电图,排除异常心电图者。将动物随机分为手术组(50只)、假手术组(25只)。手术组根据术后心电图不同分为SAH组、CCS组,每组各25只(不足者同条件新鼠补入),各组大鼠按取材时段不同随机分为1、3、5、7、14 d等5个亚组,每组5只。造模后用Power Lab系统检测心电图,分别于1、3、5、7、14 d再次检测心电图,用酶联免疫吸附测定(ELISA)法检测血浆中肾素活性(PRA)及血管紧张素Ⅱ(AngⅡ)的含量。结果造模成功后,假手术组心电图未见明显变化,手术组术后2 h CCS病发率最高。1 d时SAH组、CCS组PRA、AngⅡ含量均高于假手术组(P<0.05),且CCS组高于SAH组(P<0.05);SAH组和CCS组PRA、AngⅡ在3 d时达到高峰,显著高于假手术组(P<0.05),而CCS组高于SAH组(P<0.05);5、7 d时SAH组、CCS组PRA、AngⅡ含量逐渐下降,仍高于假手术组(P<0.05)。假手术组PRA、AngⅡ含量随时间延长逐渐下降至7 d后趋于稳定;SAH组及CCS组PRA、AngⅡ含量3 d达高峰,后逐渐下降至14 d基本趋于假手术组水平(P>0.05)。结论 SAH并发CCS的PRA、AngⅡ呈逐渐上升达高峰后逐渐下降趋于稳定的变化,提示PRA、AngⅡ在SAH并发CCS的发生与发展中起到了一定的作用。

Objective To investigate the changes of renin-angiotensin in rats with subarachnoid hemorrhage（SAH）complicated by cerebral-cardiac syndrome（CCS）. Methods The occipital large pool was injected with autologous arterial blood to replicate the SAH model. Seventy-five healthy male Wistar rats were 6 weeks old and weighed 150-200 g.All rats were connected to a dynamic laboratory system（Power Lab system） to detect ECG, excluding abnormal ECG.The rats were randomly divided into surgery group（50 rats） and sham group（25 rats）. The surgical group was divided into SAH group and CCS group according to different electrocardiograms after surgery. Each group had 25 animals（insufficient ones were recruited in the same condition as new rat）. Different time periods were randomly divided into five subgroups： 1, 3, 5, 7, 14 d group, with 5 rats in each group. Electrocardiograms were measured with the Power Lab system after modeling, and electrocardiograms were again detected on day 1, 3, 5, 7 and 14. The levels of plasma renin activity（PRA） and angiotensin Ⅱ（AngⅡ） were detected by enzyme-linked immunosorbent assay（ELISA）. Results After successful modeling, there was no significant change in electrocardiogram in the sham-operated group. The incidence of cerebral-cardiac syndrome was highest in the surgical group at 2 hours after surgery. The levels of PRA and Ang Ⅱin SAH group and CCS group were higher than those in sham-operated group at day 1（P〈0.05）, and the CCS group was higher than the SAH group（P〈0.05）; PRA and AngⅡ peaked at 3 days in SAH group and CCS group were significantly higher than that in sham operation group（P〈0.05）, and CCS group was higher than the SAH group（P〈0.05）. The contents of PRA and Ang Ⅱ in SAH group and CCS group decreased gradually after 5 and 7 days, which was still higher than that in sham operation group（P〈0.05）. The contents of PRA and AngⅡ gradually decreased to 7 days and then stabilized in the sham operation group. The levels of PRA and Ang Ⅱ in the SAH group and the CCS group reached the peak at 3 days, and then gradually decreased to 14 days afterwards, and the levels of PRA and AngⅡ basically reached the level of the sham operation group（P〉0.05）. Conclusion PRA and AngⅡ in SAH complicated with CCS gradually increase and reach a peak, then gradually decrease and tend to be stable. This suggests that PRA and AngⅡ play a certain role in the occurrence and development of SAH concurrent with CCS.