不同人参皂苷为标准品测定皂苷含量的比较研究

Comparative Study on Determination of Saponin Content by Different Ginsenosides as Standard Product

人参皂苷是显示人参根和根茎的生物及药理活性、特征的主要成分。其中,二醇组人参皂苷含量高达四分之三。测定皂苷的方法因类而宜,每一种方法都有其局限性。目前国标方法中采用比色法并以三醇组人参皂苷R_e为标准品进行人参总皂苷含量测定。试验通过比色法,分别以R_e和二醇组人参皂苷R_C为标准品,对二醇组人参总皂苷、三醇组人参总皂苷和人参茎叶总皂苷的皂苷含量进行检测。以R_e为标准品计算出的三种样品的人参总皂苷的平均含量,分别为0.341 8,0.581 9和0.550 7 mg;以R_C为标准品的含量分别为0.297 9,0.521 0和0.491 9 mg。以R_e为标准品计算出的总人参皂苷含量比R_C高。加标准品R_C比R_e所测的回收率高。试验同时进行了稳定性、精密度和重复性检测,该方法简便、准确、重复性好。用不同标品检测人参总皂苷含量存在误差,可用两种标品进行检测,取平均值更具有意义。

Ginsenosides are the main components that show the biological and pharmacological activity of ginseng roots and rhizomes. Among them, the protopanaxadiol content is up to three quarters. The method of determining saponins is due to the fact that each method has its limitations. The current method of GB using colorimetric method and ginsenoside R_e of protopanaxatriol is used as the standard for ginsenosides content determination. In this study, we tested the ginseng total saponins concentration of panaxadiol saponins, panaxatriol saponins, ginseng stem and leaf saponins which were detected by ginsenosides R_e and R_C as the standard. The average concentration of total ginsenosides calculated by ginsenoside R_e as the standard was 0.341 8, 0.581 9 and 0.550 7 mg, respectively. R_C as the standard was 0.297 9, 0.521 0 and 0.491 9 mg, respectively. The total saponins concentration calculated from R_e standard was higher than that of R_C. The addition of standard R_C had a higher recovery than R_e. The test was conducted for stability, precision and repeatability testing, they show that the method is simple, accurate and reproducible. With different standard products to detect total concentration of ginseng saponins error, we can be used to detect two kinds of standard and the average is more meaningful.