β1-肾上腺素能受体基因编码区1165位单核苷酸多态性与肠道病毒A71感染的关系

Association between the single nucleotide polymorphism at locus 1165 of β1-adrenoceptor and infection by Enterovirus A71

目的探讨β1-肾上腺素能受体（β1-adrenoceptor, β1-AR）基因编码区1 165位单核苷酸多态性（single nucleotide polymorphism, SNP）与肠道病毒A71（enterovirus A71, EV-A71）感染的关系。方法采用PCR体外扩增技术，通过对PCR产物进行Sanger测序，检测EV-A71手足口病（hand, foot and mouth disease, HFMD）患儿和健康对照者的β1-AR基因编码区1 165位SNP基因型，采用卡方检验比较EV-A71感染组与健康对照组β1-AR基因编码区1 165位SNP基因型频率的差异。 结果77例EV-A71 HFMD患儿和66名健康儿童存在G1165C多态及GG、GC、CC 3种基因型，在EV-A71 HFMD患儿中GG、GC、CC基因型频率分别为10%、47%、43%，G、C等位基因频率分别为34%、66%，在健康儿童中GG、GC、CC基因型频率分别为7%、41%、52%，G、C等位基因频率分别为28%、72%。经卡方检验分析，EV-A71感染组与健康对照组GG、GC、CC基因型频率分布差异无统计学意义（χ2＝1.154, df＝2, P＝0.562），G、C等位基因频率分布差异无统计学意义（χ2＝1.091, df＝2, P＝0.296）；EV-A71感染轻症组和重症组GG、GC、CC基因型频率分布差异无统计学意义（χ2＝3.945, df＝2, P＝0.139）；G、C等位基因频率分布差异无统计学意义（χ2＝3.763, df＝2, P＝0.052）。结论β1-AR基因编码区1 165位SNP与EV-A71感染及其严重程度无关。

ObjectiveTo detect the single nucleotide polymorphism （SNP） at locus 1 165 of β1-adrenoceptor （β1-AR） and to investigate the association between the SNP and the infection by enterovirus A71（EV-A71）.MethodsPolymerase chain reaction （PCR） amplification technique was used to detect the SNP at locus 1 165 of β1-AR between hand, foot and mouth disease （HFMD） and healthy controls by sanger sequencing method .ResultsThere was a G1165C SNP and three kinds of genotypes （GG, GC, CC） in β1-AR gene in the 77 cases of EV-A71 HFMD patients and 66 cases of healthy controls. For HFMD patients, frequencies of GG, GC and CC genotypes of the G1165C locus were 10%, 47% and 43%, respectively, and alleles frequency of G and C were 34% and 66%, respectively. But in healthy children, GG, GC, CC genotype frequencies were 7%, 41% and 52%, respectively, and G and C allele frequencies were 28% and 72% respectively. Chi-square analysis showed that there were no significant differences in distribution of genotypes （χ2=1.154, df=2, P=0.562） and alleles frequency （χ2=1.091, df=2, P=0.296） between the EV-A71-infected group and the healthy control group. Between mild and severe EV-A71-infected group, there were no significant differences in distribution of genotypes （χ2=3.945, df=2, P=0.139） and alleles frequency （χ2=3.763, df=2, P=0.052）.ConclusionsThe 1 165 SNP in the coding region of β1-AR was not associated with EV-A71 infection and its severity.