基于网络药理学的降脂理肝汤治疗非酒精性脂肪肝病作用机制研究

Action mechanism of Jiangzhi Ligan Decoction in treatment of non-alcoholic fatty liver disease based on network pharmacology

目的筛选降脂理肝汤主要活性成分,预测其活性成分的作用靶点,探讨降脂理肝汤治疗非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)的多成分-多靶点-多通路作用机制。方法通过多个数据库联合检索并筛选降脂理肝汤活性成分和其作用靶点以及NAFLD相关靶点,并预测降脂理肝汤治疗NAFLD的潜在靶点。采用Cytoscape软件构建降脂理肝汤活性成分-作用靶点网络,通过Cytoscape的插件Clue GO对靶点基因功能及通路进行富集分析。结果网络分析结果表明,从降脂理肝汤中筛选得到82个活性成分,涉及NAFLD靶点53个,靶点主要关联NAFLD中胰岛素抵抗、氧化应激和炎症反应,并且参与了NAFLD中PI3K/Akt信号通路、炎症小体途径、白细胞介素-10(IL-10)信号通路以及T细胞信号通路,这些生物过程和信号通路介导了降脂理肝汤干预NAFLD潜在机制。结论为进一步深入研究降脂理肝汤治疗NAFLD作用机制提供了重要依据。

Objective To characterize the ＂multi-components, multi-targets, and multi-pathways＂ mechanism of Jiangzhi Ligan Decoction on non-alcoholic fatty liver disease（NAFLD） using network pharmacology technology. Methods Data regarding natural molecules of Jiangzhi Ligan Decoction, targets of NAFLD, and interactions between natural molecules and NAFLD targets were screened. Network pharmacology of the interactions between natural molecules and NAFLD targets were established using Cytoscape software. The biological process and the signaling pathway of the putative targets were analyzed using Clue GO. Results The network analysis indicated that 82 active ingredients and 53 NAFLD related targets were screened in Jiangzhi Ligan Decoction, which was involved in regulation of insulin resistance, oxidative stress, inflammation, PI3 K/Akt signaling pathway, inflammasome signaling pathway, IL-10 signaling pathway, and T cell signaling pathway. Conclusion This study provides an important basis for further study on the pharmacological mechanism of Jiangzhi Ligan Decoction in the treatment of non-alcoholic fatty liver disease.