冰片联合紫杉醇对脑胶质瘤细胞的化疗增效作用

Chemo-sensitization Effect of Borneol Combined with Paclitaxel on Brain Glioma Cell Lines

目的:探讨冰片(Bor)对紫杉醇(PTX)抑制脑胶质瘤细胞(U87)增殖的增效作用及其相关机制。方法:以体外培养的脑胶质瘤细胞为研究对象,采用四甲基偶氮唑蓝(MTT)和ATP检测法检测细胞增殖和凋亡情况,检测活性氧(ROS)水平和罗丹明(Rh123)浓度变化。结果:冰片能促进紫杉醇的抗肿瘤效果,增加其凋亡程度,增加ROS水平和细胞内的Rh123蓄积,与对照组比较差异有统计学意义(P<0.05)。结论:冰片具有增强PTX抗脑胶质瘤细胞增殖和凋亡的能力,此作用可能与抑制P-gp和增强ROS的水平相关。

Objective：To investigate the anti-proliferation effect of borneol（Bor）combined with paclitaxel（PTX）on brain glioma cell lines（U87）and to explore the mechanism of the chemo-sensitization effect.Methods：MTT and Intracellular ATP level assay were used to detect the cell viability rate and apoptosis of brain glioma cells.Then we detected the ROS content and cellular accumulation of Rho123.Results：The study showed that Borneol combined with paclitaxel could enhance the anti-proliferation,reduce the intracellular ATP levels,increase the ROS content and cellular accumulation of Rho123,compared with PTX alone.Conclusion：The results shows that Borneol could inhibit the proliferation and strengthen apoptosis of U87,which could have relationship with downregulating the expression of P-gp and enhancing the ROS level.