摘要
目的:探讨冰片(Bor)对紫杉醇(PTX)抑制脑胶质瘤细胞(U87)增殖的增效作用及其相关机制。方法:以体外培养的脑胶质瘤细胞为研究对象,采用四甲基偶氮唑蓝(MTT)和ATP检测法检测细胞增殖和凋亡情况,检测活性氧(ROS)水平和罗丹明(Rh123)浓度变化。结果:冰片能促进紫杉醇的抗肿瘤效果,增加其凋亡程度,增加ROS水平和细胞内的Rh123蓄积,与对照组比较差异有统计学意义(P<0.05)。结论:冰片具有增强PTX抗脑胶质瘤细胞增殖和凋亡的能力,此作用可能与抑制P-gp和增强ROS的水平相关。
Objective:To investigate the anti-proliferation effect of borneol(Bor)combined with paclitaxel(PTX)on brain glioma cell lines(U87)and to explore the mechanism of the chemo-sensitization effect.Methods:MTT and Intracellular ATP level assay were used to detect the cell viability rate and apoptosis of brain glioma cells.Then we detected the ROS content and cellular accumulation of Rho123.Results:The study showed that Borneol combined with paclitaxel could enhance the anti-proliferation,reduce the intracellular ATP levels,increase the ROS content and cellular accumulation of Rho123,compared with PTX alone.Conclusion:The results shows that Borneol could inhibit the proliferation and strengthen apoptosis of U87,which could have relationship with downregulating the expression of P-gp and enhancing the ROS level.
作者
郭源源
王鹏云
Guo Yuanyuan;Wang Pengyun(Department of Pharmacy,Liyuan Hospital,Tongji Medical School,Huazhong University of Science and Technology,Wuhan 430077,China;Laboratory Medicine,Liyuan Hospital,Tongji Medical School,Huazhong University of Science and Technology,Wuhan 430077,China)
出处
《亚太传统医药》
2018年第9期9-11,共3页
Asia-Pacific Traditional Medicine
基金
国家自然科学基金青年基金(81601589)
湖北省卫生和计划生育委员会青年人才项目(WJ2017Q026)
关键词
冰片
紫杉醇
脑胶质瘤细胞
细胞增殖
Borneol
Paclitaxel
Brain Glioma Cells
Cell Proliferation