摘要
病原菌对抗生素的耐受对临床治疗造成严重威胁。CRISPR-Cas系统具有高度的靶标基因序列特异性与方便的可编程性,已被尝试用来控制耐药病原细菌。用噬菌体传递CRISPR-Cas系统切割耐药细菌特有靶标,可恢复其对抗生素的敏感或直接致其死亡。更吸引人的应用则是用CRISPR-Cas系统编辑噬菌体基因组,使其具备期望的抗菌性能。
The tolerance of pathogens to antibiotics poses a serious threat to clinical treatment. The CRISPR-Cas system has a high specificity of target gene sequence and convenient programmability and has been tried to control drug-resistant pathogenic bacteria. The use of the phage-delivering CRISPR-Cas system to cleave specific targets of resistant bacteria restores sensitivity to antibiotics or directly causes death. A more attractive application is to use the CRISPR-Cas system to edit the phage genome for desired antibaial propertiecters.
作者
杨萍
孙兵兵
杨俊杰
杨晟
蒋宇
Yang Ping;Sun Bing-bing;Yang Jun-jie;Yang Sheng;Jiang Yu(School of Pharmacy,Fudan University,Shanghai 201203;Shanghai Institute of Pharmaceutical Industry,Shanghai 201203;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240;Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032)
出处
《中国抗生素杂志》
CAS
CSCD
2018年第8期927-931,共5页
Chinese Journal of Antibiotics
基金
国家自然科学基金面上项目(No.81573329)
