兔儿伞化学成分研究

Chemical constituents from Syneilesis aconitifolia

目的研究兔儿伞Syneilesis aconitifolia全草的化学成分。方法利用硅胶柱色谱、制备液相色谱、重结晶等方法进行分离、纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果从兔儿伞全草甲醇提取物正己烷萃取部位分离得到9个化合物,分别鉴定为3-(2-甲基-2-丁烯酰氧基)-8-甲氧基-6,15-环氧-艾里莫芬-7(11)-烯-12,8α-内酯(1)、β-芹子烯(2)、(-)-ent-6α-甲氧基桉叶-4(15)-烯-1β-醇(3)、5α,7αH-桉叶-3-烯-15-醛-1-酮(4)、大根香叶-1β-醇(5)、10α-羟基日本刺参萜-4-酮(6)、10β-羟基-6β,8β-二甲氧基-艾里莫芬-7(11)-烯-12,8α-内酯(7)、8β,10β-羟基-6β-甲氧基艾里莫芬-7(11)-烯-12,8α-内酯(8)、4(15)-烯-桉叶-1β,6α-二醇(9)。并采用MTT法对化合物1、3、5、6、8、9的人宫颈癌He La细胞毒活性进行体外筛选。结论化合物1为未见报道的新倍半萜化合物,命名为兔儿伞内酯A,化合物2~5、7为首次从兔儿伞中分离得到。化合物1、5、6、8、9均对He La细胞具有一定的抑制作用。

Objective To study the chemical constituents from Syneilesis aconitifolia. Methods The chemical constituents were separated and purified by silica gel column chromatography and HPLC. Their structures were determined based on spectroscopic analyses. Results Nine sesquiterpenes compounds were isolated and the structures were identified as 3-angeloyloxy-8-methoxy-6,15-epoxy-eremophil-7（11）-en-12,8α-olide（1）, β-selinene（2）,（-）-ent-6α-methoxyeudesm-4（15）-en-1β-ol（3）, 1-oxo-5α,7αH-eudesma-3-en-15-al（4）, germacrane alcohol（5）, oplopanone（6）, 6β,8β-dimethyoxy-10β-hydroxyeremophil-7（11）-en-12,8α-olide（7）, 8β,10β-dihydroxy-6β-methyoxyeremophil-7（11）-en-12,8α-olide（8）, and 1β,6α-diidroxi-4（15）-eudesmeno（9）. Compounds 1, 3, 5, 6, 8, and 9 were tested for their anticancer activity against uterine cancer（He La）. Conclusion Compound 1 is a new compound, named as syneilesis lactone A. Compounds 2—5, and 7 are isolated from this plant for the first time. Compounds 1, 5, 6, 8, and 9 show inhibitory activity against He La.