黄芪多糖对小鼠MC-3T3-E1成骨细胞维生素D受体mRNA及蛋白表达的影响

Effects of astragalus polysaccharides on the expression of vitamin D receptor mRNA and protein in mouse osteoblast cells MC3T3-E1

目的探讨黄芪多糖对小鼠MC-3T3-E1成骨细胞维生素D受体(VDR)mRNA及蛋白表达的影响。方法采用Real-Time PCR、Western Blot检测黄芪多糖对小鼠MC-3T3-E1成骨细胞维生素D受体(VDR)mRNA及蛋白表达情况。实验分为5组,正常组、维生素D组(10-5mmol·L-1)、10μg·ml-1黄芪多糖组、1μg·ml-1L黄芪多糖组、0.1μg·ml-1黄芪多糖组。结果 Real-Time PCR、Western Blot检测结果显示,与正常组比较,不同剂量的黄芪多糖均可上调小鼠MC-3T3-E1成骨细胞VDR mRNA及蛋白相对表达量,且在黄芪多糖剂量为1μg·ml-1时其表达量显著增高(P<0.05)。结论黄芪多糖治疗骨质疏松症重要机制之一可能与上调VDR mRNA及蛋白表达水平有关。

Objective To investigate the effect of astragalus polysaccharide on the expression in mouse osteoblast cells MC3 T3-E1 of vitamin D receptor(VDR) mRNA and protein. Methods Real-Time PCR and Western Blot were used to detect the expression of vitamin D receptor(VDR) mRNA and protein in mouse osteoblast cells MC3 T3-E1 by astragalus polysaccharides.The experiment was divided into 5 groups: normal group,the vitamin D group(10-5 mmol · L-1),astragalus polysaccharide group(10 μg·m L-1),astragalus polysaccharide group(1 μg·m L-1 L),astragalus polysaccharide group(0. 1 μg·m L-1). Results The results of Real-Time PCR and Western Blot showed that compared with the normal group,different doses of Astragalus polysaccharides could up regulate the relative expression of VDR,mRNA and protein in the osteoblast cells MC3 T3-E1 of mouse,The expression of astragalus polysaccharide was increased significantly when the dose of Astragalus polysaccharide was 1 μg ·m L-1(P < 0. 05). Conclusion One of the important mechanisms of astragalus polysaccharide on treatment of osteoporosis may be related to the upregulation of VDR mRNA and protein expression levels.