白藜芦醇对M1/M2巨噬细胞极化的作用及其机制

Effects of Resveratrol on M1/M2 macrophage polarization

目的研究白藜芦醇对M1/M2型巨噬细胞极化以及M1/M2型巨噬细胞能量代谢的影响。方法建立了骨髓来源巨噬细胞(BMDM)的体外培养模型。在BMDM以及Raw264.7细胞中加入白藜芦醇,应用RT-q PCR以及Western blot检测M1、M2型巨噬细胞的标志分子。应用细胞外流量测试仪检测白藜芦醇M1、M2型巨噬细胞能量代谢的影响。结果白藜芦醇可以促进M2型巨噬细胞极化,抑制M1型巨噬细胞极化。白藜芦醇促进M2型巨噬细胞标志蛋白arginase1的表达,同时也会促进抗炎细胞因子IL-10的表达。LPS具有促进M1型巨噬细胞糖酵解的功能,而白藜芦醇可以抑制LPS引起的巨噬细胞糖酵解增加。结论白藜芦醇作为天然产物之一,可通过抑制NO生成进而调控M1/M2型巨噬细胞的能量代谢,促进M2型巨噬细胞的极化。

Objective To investigate the effects of resveratrol on M1/M2 macrophage polarization and M1/M2 macrophage energy metabolism. Methods Bone marrow - derived macrophage （BMDM） and Raw264.7 cells were treated with resveratrol, the mark- er of M1/M2 macrophage were determined by RT - qPCR and Western blot. The effects of resveratrol on M1 ,M2 macrophage en- ergy metabolism were analyzed by seahorse. Results In this study, Resveratrol promoted M2 macrophage polarization and inhibi- ted LPS - induced M1 macrophage polarization in BMDM and Raw264.7 cell line. Resveratrol directly increased M2 macrophage marker genes expression including argianse 1, CD163 and anti - inflammatory cytokine IL - 10 expression. Further, the level of ECAR was increased in LPS- induced M1 macrohpage analyzed by seahorse. However, resveratrol decreased LPS- induced up- regulation of ECAR. Conclusion Altogether, the current study demonstrates that resveratrol inhibited NO production induced by LPS, thereby regulated M1/M2 macrophage energy metabolism, promoted M2 macrophage polarization.