生麦芽和炒麦芽对高脂血症大鼠的降血脂作用研究

Hypolipemic Effect of Raw Hordei Fructus Germinatus and Processed Hordei Fructus Germinatus on Hyperlipidemia Mice

目的：研究生麦芽和炒麦芽对高脂血症大鼠的降血脂作用,比较生麦芽和炒麦芽降血脂作用的差异。方法：饲喂高脂饲料及灌服脂肪乳4周建立高脂血症大鼠模型,分别给予不同剂量的生麦芽和炒麦芽水煎液灌胃干预4周。造模4周后通过眼眶取血,生化法检测血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白-胆固醇（LDL-C）、高密度脂蛋白-胆固醇（HDL-C）的含量。灌胃4周后,称量大鼠体质量及肝脏重量,检测血清TG、TC、LDL-C、HDL-C含量,计算动脉硬化指数（AI）,比色法检测肝组织匀浆中的丙二醛（MDA）水平以及超氧化物歧化酶（SOD）活性。HE染色观察肝组织病理学变化。结果：造模4周后,与正常对照组比较,模型对照组大鼠的TG、TC、LDL-C、AI水平升高、HDL-C水平降低。灌胃4周后,与模型对照组比较,辛伐他汀组、生麦芽和炒麦芽水煎液组对大鼠体质量无明显影响,辛伐他汀组、生麦芽和炒麦芽能够显著降低肝指数（P〈0.05或P〈0.01）,辛伐他汀组、生麦芽和炒麦芽都能够降低高脂血症大鼠血清TC、TG、LDL-C、AI值（P〈0.05或P〈0.01）,升高HDL-C值（P〈0.05或P〈0.01）,降低肝脏组织MDA水平（P〈0.05或P〈0.01）,升高SOD水平（P〈0.05）。结论：生麦芽和炒麦芽对高脂血症大鼠具有降血脂作用,且生麦芽效果强于炒麦芽。

Objective：To study the hypolipemic effect of Hordei Fructus Germinatus and processed Hordei Fructus Germinatus on hyperlipidemia mice and compare the differences in hypolipemic effect between raw Hordei Fructus Germinatus and processed Hordei Fructus Germinatus. Methods：The rats were given the high-fat feeds to induce hyperlipidemia model. The weight was observed. TG,TC,LDL-C,HDL-C,ALT and AST in serum of rats were tested. MDA and T-SOD levels of the liver tissue were tested simultaneously. Histopathological changes of liver were observed by HE staining. Results：Compared with the model group,the levels of TG,TC and LDL-C were reduced significantly in raw Hordei Fructus Germinatus and processed Hordei Fructus Germinatus（ P〈0.05 or P〈0.01）,while HDL-C levels increased significantly. In the liver tissue,MDA level was significantly decreased（ P〈0.05 or P〈0.01）,while SOD level was increased（ P〈0.05） obviously. Conclusion：Raw Hordei Fructus Germinatus and processed Hordei Fructus Germinatus had hypolipidemic effect and raw Hordei Fructus Germinatus was better than the processed Hordei Fructus Germinatus.