顶空固相微萃取-气相色谱-质谱联用分析不同品牌艾柱及艾条挥发性成分

A comparative analysis of volatile components of moxa cone and stick from different manufacturers using HS-SPME-GC-MS

目的分析不同品牌艾柱及艾条中挥发性成分。方法采用顶空固相微萃取-气相色谱-质谱(HS-SPME-GCMS)联用技术,对不同品牌艾柱及艾条中挥发性成分进行定性分析,并以峰面积归一化法计算各组分的相对含量。实验数据通过主成分分析进行综合评价。结果 5个厂家生产的7个批次艾柱及艾条挥发性成分的总离子流图整体上相似,但挥发性成分有所差异。初步鉴定出了86种化合物,检测出含量较高的共有成分有桉油精、樟脑、松油醇、冰片乙酸酯、l-石竹烯、氧化石竹烯、(-)-4-萜品醇、崖柏酮等。结论运用HS-SPME-GC-MS能快速获取艾柱及艾条的挥发性成分的化学组成信息,不同品牌艾柱及艾条中的挥发性成分存在明显差异。

Objective To analyze the volatile components in different brands of moxa cones and sticks. Methods Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry（HS-SPME-GC-MS）was used to qualitatively analyze volatile components in different brands of moxa cones and sticks. The relative content of each component was calculated by peak area normalization method. The experimental data were comprehensively evaluated by principal component analysis. Results The total ion chromatograms of the volatile components of moxa cones and sticks in 7 batches produced by 5 manufacturers were similar on the whole,but the volatile components were still different. Eighty-six compounds were identified initially. The most common components were found to be eucalyptol,camphor,terpineol,bornyl acetate,caryophyllene,caryophyllene,oxide（-）-4-terpineol,and cliff ketone. Conclusion Using HS-SPME-GC-MS could quickly provide information for the chemical composition of the volatile components in the moxa cones and sticks,and there are significant differences in the volatile components among different brands.