改良ELISA法定量测定血清中段心房利钠肽原的评价及临床应用

Evaluation and Clinical Application of Modified Enzyme-Linked Immunosorbent Assay for Quantitative Determination of Midregional Pro-Atrial Natriuretic Peptide in Serum

目的建立血清中段心房利钠肽原(MR-proANP)的改良ELISA法定量测定,并探讨其应用价值。方法用定量ELISA法测定患者与健康者的血清MR-proANP,并运用IBM SPSS Statistics 23.0软件对结果进行统计处理。结果该法检出限为1.002pmol/L,平均回收率为98.33%,批内CV为2.64%,批间CV为4.95%。健康者与患者的血清MRproANP值通过对数转换,并用均数±标准差(珚x±s)表示;男性健康者120例、高血压41例、急性心肌梗死(AMI)23例、心功能衰竭37例及获得性肺炎患者41例的血清MR-proANP值分别为1.25±0.48,2.45±0.34,3.10±0.41,2.73±0.51和2.43±0.34pmol/L,差异具有统计学意义(F=166.487,P=0.000);女性健康者120例、高血压40例、AMI 22例、心功能衰竭35例及获得性肺炎患者39例的血清MR-proANP值分别为1.39±0.48,2.73±0.59,3.47±0.69,3.06±0.75和2.70±0.58pmol/L,差异也具有统计学意义(F=122.412,P=0.000)。结论该法灵敏、特异和准确。血清MR-proANP定量测定是诊断高血压、AMI,心功能衰竭和获得性肺炎的较好实验指标。

Objective To establish enzyme-linked immunosorbent assay（ELISA）for the quantitative determination of midregional pro-atrial natriuretic peptide（MR-pro ANP）in serum and to investigate its clinical application.Methods Serum MRpro ANP concentrations of patients and of normal controls were measured by ELISA.IBM SPSS Statistics 23.0 software was employed for statistical analysis in this study.Results The limit of detection（LOD）was 1.002 pmol/L.The average rate of recovery was 98.33%.The intraassay CV was 2.64% and interassay CV was 4.95%,respectively.The serum MRpro ANP value of healthy person and patient were transformed through logarithmic transformation,expressed in Mean±SD.The male value of healthy persons（n=120）,hypertension（n=41）,AMI（n=23）,heart failure（n=37）and acquired pneumonia（n=41）were 1.25±0.48,2.45±0.34,3.10±0.41,2.73±0.51 and 2.43±0.34 pmol/L,respectively.Significant differences were observed in them（F=166.487,P=0.000）.The female value were 1.39±0.48,2.73±0.59,3.47±0.69,3.06±0.75 and 2.70±0.58 pmol/L,respectively.Significant differences were observed in them（F=122.4,P=0.000）.Conclusion This method is sensitive,specific and accurate.Quantitative detection of MR-pro ANP is now better laboratory test for patients of hypertension,of AMI,of heart failure and of acquired pneumonia.