HBV感染并发血小板减少患者外周血中CD4^+CD25^+调节性T细胞的表达研究

Expression of CD4^+CD25^+ Regulatory T Cells in Peripheral Blood of Patients with Hepatitis B Virus Infection with Thrombocytopenia

目的探讨CD4^+CD25^+调节性T细胞与乙肝病毒(HBV)感染并发血小板减少发病的关系及可能机制。方法收集2017年3~11月在桂林医学院附属医院确诊的12例HBV感染并发免疫性血小板减少症(ITP)患者和同期体检的10例健康个体外周血,分别作为实验组和对照组,应用流式细胞术检测外周血中CD4^+/淋巴细胞、CD4^+CD25^+/淋巴细胞及CD4^+CD25^+/CD4^+比例。结果实验组与对照组CD4^+/淋巴细胞、CD4^+CD25^+/淋巴细胞、CD4^+CD25^+/CD4^+比例分别为(24.64+9.8)%vs(31.72+6.6)%,(4.05+3.7)%vs(3.61+1.2)%和(14.97+12.0)%vs(11.27+2.8)%,差异均无统计学意义(U=33.0,51.5,55.0,P=0.075,0.575,0.742)。结论 CD4^+CD25^+调节性T细胞在HBV感染并发血小板减少患者外周血中比例与正常人无明显差异,可能是HBV感染相关免疫耐受与ITP相关免疫调节异常共同作用的结果。

Objective To investigate the relationship between CD4＋CD25＋regulatory T cells（Tregs）and the pathogenesis of hepatitis B virus（HBV）infection with thrombocytopenia and its possible mechanism.Methods Peripheral blood of 12 patients diagnosed as HBV infection with thrombocytopenia and 10 healthy donors at the same time were collected from the Affiliated Hospital of Guilin Medical College from March to November,2017 and classified as experimental and controlled groups respectively.Flowcytometry was used to evaluate the proportion of CD4＋/T cells,CD4＋CD25＋/T cells and CD4＋CD25＋/CD4＋.Results The proportions of CD4＋/T cells,CD4＋CD25＋/T cells and CD4＋CD25＋/CD4＋in experimental and controlled group respectively were[（24.64＋9.8）% vs（31.72＋6.6）%,U =33.0,P=0.075],[（4.05＋3.7）% vs（3.61＋1.2）%,U=51.5,P=0.575]and[（14.97＋12.0）% vs（11.27＋2.8）%,U=55.0,P=0.742]with no statistical significance.Conclusion There was no significant difference between patients of HBV infection with thrombocytopenia and the normal controls in the proportion of CD4＋CD25＋regulatory T cells in peripheral blood.It may be the result of HBV immune related immune tolerance and immune regulatory abnormalities associated with immune thrombocytopenia（ITP）.