肝癌组织AKR1B10的表达与HBV感染和预后的关系研究

Expression of AKR1B10 in liver cancer tissue and its relationship with HBV infection and prognosis

目的研究肝癌组织中醛糖还原酶相似蛋白(AKR1B10)的表达及与乙型肝炎病毒(HBV)感染和预后的关系。方法选取2013年2月-2015年5月医院收治的原发性肝癌患者110例为研究对象,根据HBV感染与否将所有患者分为感染组65例与非感染组45例。分别采集所有患者术前血清,并检测其中HBV-DNA与甲胎蛋白的水平;同时,检测患者肝癌组织中AKR1B10的表达情况;分析肝癌组织中AKR1B10表达情况与临床病理指标关系,比较感染组与非感染组肝癌组织中AKR1B10表达情况,分析肝癌组织中AKR1B10表达情况与术后复发的关系。结果甲胎蛋白水平为阳性者肝癌组织中AKR1B10高表达发生率为55.36%,低于甲胎蛋白水平阴性的90.74%(P<0.05);感染组肝癌组织AKR1B10低表达占35.38%,高于非感染组的15.56%(P<0.05);肝癌组织中AKR1B10低表达患者术后1年复发率与术后2年复发率分别为66.67%、76.67%,均高于肝癌组织中AKR1B10高表达患者的35.00%、51.25%(P<0.05)。结论肝癌组织中AKR1B10的表达与甲胎蛋白水平、HBV感染有关,临床上可通过对AKR1B10的表达情况进行检测,从而有助于判断患者甲胎蛋白水平与HBV感染情况,同时可为患者预后评估提供指导作用。

OBJECTIVE To study the expression of aldo-keto reductase family1,member B10（AKR1 B10）in liver cancer tissue and its relationship with hepatitis B virus（HBV）infection and prognosis.METHODS A total of 110 patients with primary liver cancer who were treated in Wuhan Central Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from February 2013 to May 2015 were selected as the research subjects.According to the infection of HBV,all the patients were divided into infected group with 65 cases and non-infected group with 45 cases.The serum samples of all the patients were collected before surgery and the levels of HBV-DNA and alpha fetoprotein were determined.At the same time,the expression of AKR1 B10 in liver cancer tissue was examined,and the relationship between AKR1 B10 expression and clinicopathological indices was analyzed.The expression of AKR1 B10 was compared between the infected group and the non-infected group,and the relationship between the expression of AKR1 B10 and postoperative recurrence was analyzed.RESULTS The proportion of high expression of AKR1 B10 in liver cancer tissues with positive alpha-fetoprotein was 55.36%,which was significantly lower than 90.74% of those with negative alpha-fetoprotein（P〈0.05）.The proportion of low expression of AKR1 B10 in liver cancer tissues of the infected group was 35.38%,which was significantly higher than 15.56% of the non-infected group（P〈0.05）.The 1-year and 2-year recurrence rates of patients with low expression of AKR1 B10 in liver cancer tissues after operation were 66.67% and 76.66%,respectively,which were significantly higher than 35.00% and 51.25% of patients with high expression of AKR1 B10 in liver cancer tissues（P〈0.05）.CONCLUSIONThe expression of AKR1 B10 in liver cancer tissues is closely related to the level of alpha-fetoprotein and HBV infection.In clinic,it is possible to determine the expression of AKR1 B10,which is helpful for judging the level of alpha fetoprotein and HBV infection,and it can also provide guidance for prognosis evaluation of patients.