Effect of electroacupuncture on the expression of PGC-1α and UCP-1 in the brown adipose tissue of obese rats

电针对肥胖大鼠棕色脂肪组织中PGC-1α、UCP-1蛋白表达的影响（英文）

Objective： To explore the effect of peroxisome proliferators-activated receptor γ coactivator-1α（PGC-1α） and uncoupling protein-1（UCP-1） in the brown adipose tissue（BAT） of obese rats in the process of acupuncture treatment for obesity.Methods： Fifty clean-grade male Wistar rats with the age of 3 months were randomly divided into highfat diet group（n = 40） and normal diet group（control group, n = 10）. Nutritional obesity animal models were established through feeding with high-fat diet. Twenty-four animal models in the high-fat diet group were established successfully, and then they were randomly divided into model group, acupuncture group and non-acupoint group,with 8 rats in each group. In acupuncture group, Zusanli（足三里ST36） and Tianshu（天枢ST 25） were selected. In non-acupoint group,the non-acupoints located on 5 mm beside ST 36 and ST 25 were selected as acupuncture points, and electroacupuncture intervention was adopted for 5 times/week for 8 weeks. The body mass of obese rats was measured, the body fat ratio in BAT of rats was calculated, and the expression levels of PGC-1α and UCP-1 in BAT of rats were detected by immunohistochemical method.Results： ① After treatment, the body mass of rats in acupuncture group reduced significantly, which increased in the other three groups. The changing value of body mass of rats in acupuncture group was higher than that in model group（P 0.05）, the changing value of body mass of rats in acupuncture group was higher than that in non-acupoint group（P 0.05） and the difference in body mass changing value of rats between non-acupoint group and model group was not statistically significant（P 0.05）,the body fat ratio in BAT of rats in non-acupoint group was lower than that in acupuncture group, and the differences were statistically significant（P0.05）. ② Compared with model group, the body fat ratio in BAT of rats in acupuncture group increased significantly（P 0.05）,and the difference in body fat ratio in BAT of rats between non-acupoint group and model group was not statistically significant（P 0.05）.③ Compared with model group, the PGC-la and UCP-1 levels in BAT of obese rats in acupuncture group increased（P 0.05）, and the difference in expression levels of PGC-la and UCP-1 in BAT of rats between non-acupoint group and model group was not statistically significant（P 0.05）, the expression levels in non-acupoint group were lower than that in acupuncture group, and the differences were statistically significant（P 0.05）.Conclusion： Electroacupuncture at ＂ST 36＂ and ＂ST 25＂ can effectively up-regulate the expression levels of PGC-la and UCP-1 of diet induced obesity rats, it indicates that ＂ST 36＂ and ＂ST 25＂ have close relationship with obesity and it is may be one of the effect mechanisms of electroacupuncture in losing weight through facilitating the ＂browning reaction＂ of white adipose tissue.

目的：探讨大鼠棕色脂肪组织（brown adipose tissue,BAT）中过氧化物酶体增殖物活化受体γ辅助活化因子1α（peroxisome proliferatorsactivated receptorγcoactivator-1α,PGC-1α）以及解耦联蛋白（uncoupling protein-1,UCP-1）在针刺减肥中的作用。方法：清洁级三月龄雄性Wistar大鼠50只随机分为高脂组（40只）和普食组（即对照组,10只）,以高脂饲料喂养建立营养型肥胖动物模型。高脂组中造模成功的24只实验大鼠随机分为三组,模型组、针刺组和非穴位组,每组各8只。针刺组取＂足三里＂＂天枢＂穴,非穴位组取＂足三里＂＂天枢＂穴旁开5 mm区域的非穴位区作为针刺点,采用电针干预,5次/周,共8周。测量大鼠体质量,计算BAT的脂体比,免疫组化法检测大鼠BAT中PGC-1α和UCP-1蛋白表达。结果：（1）治疗后,针刺组大鼠体质量显著降低,其余三组大鼠的体质量均有所增加。且针刺组大鼠体质量变化值高于模型组（P〈0.05）,针刺组大鼠体质量变化值显著高于非穴位组（P〈0.05）,非穴位组大鼠体质量变化值与模型组大鼠比较差异无统计学意义（P〉0.05）。（2）与模型组比较,针刺组大鼠的BAT的脂体比明显增加（P〈0.05）,非穴位组大鼠的BAT的脂体比差异无统计学意义（P〉0.05）,非穴位组大鼠的BAT的脂体比低于针刺组,差异有统计学意义（P〈0.05）。（3）与模型组比较,针刺组大鼠BAT中PGC-1α、UCP-1蛋白含量升高（P〈0.05）,非穴位组大鼠BAT中PGC-1α、UCP-1蛋白表达差异无统计学意义（P〉0.05）,非穴位组该蛋白表达低于针刺组,差异有统计学意义（P〈0.05）。结论：电针＂足三里＂、＂天枢＂穴能有效上调高脂饮食诱导的肥胖大鼠BAT中PGC-1α、UCP-1蛋白的表达,提示＂足三里＂、＂天枢＂穴与肥胖症关系密切,可能是是电针通过促进白色脂肪组织＂棕色化＂以减轻体重的效应机制之一。