婴儿血液、尿液和产妇母乳巨细胞病毒DNA检测结果的相关性

The relationship between infantile blood,urine and breast milk of human cytomegalovirus DNA test results and its clinical significance

目的探讨婴儿血液、尿液及其母亲母乳的人巨细胞病毒(HCMV)DNA检测结果的相关性及婴儿HCMV感染的诊断价值。方法收集258例婴儿血液、尿液和对应母乳的HCMV标本,采用全自动荧光定量PCR法进行检测。结果 258例婴儿血液、尿液及其母亲母乳的HCMV DNA阳性率分别为28.68%、48.06%、61.24%,血液和尿液联合检测HCMV DNA阳性率为55.04%,3种标本联合检测HCMV DNA阳性率为74.41%。3种标本HCMV DNA检测结果具有相关性,但差异无统计学意义(χ_(乳-血)~2=12.84,P=0.219;χ_(乳-尿)~2=29.02,P=0.318;χ_(尿-血)~2=63.09,P=0.444)。结论婴儿血液、尿液及其母亲母乳联合检测可能对婴儿相关疾病溯源和HCMV感染起到互补诊断,具有重要的临床意义。

Objective To analyze the relationship between infantile blood,urine and breast milk of human cytomegalovirus DNA test results,and investigate the value of three kinds of samples in the diagnosis of infantile cytomegalovirus infection. Methods The DNA of human cytomegalovirus was detected by the automatio fluorescence quantitative PCR method. Results The cytomegalovirus DNA positive rates of 258 infants with blood,urine and corresponding breast milk were 28.68%,48.06% and 61.24% respectively,the combined test of infantile blood and urine was 55.04%,the positve rate was 74.41% for three samples.Through correlation analysis and differential testing（ χ 2 milk-blood =12.84, P =0.219; χ 2 milk-urine =29.02, P =0.318; χ 2 blood-urine =63.09, P =0.444）,the DNA test results of three samples of cytomegalovirus were related,but the relationship was not close. Conclusion The combined detection of infantile blood,urine and breast milk may be of great clinical significance for the diagnosis of infant related diseases and the complementary diagnosis of cytomegalovirus infection.