日立HITACHI 7180生化检测体系的抗干扰研究

Research on anti-interference on Hitachi HITACHI 7180 test system

目的基于该实验室检测体系日立HITACHI 7180生化仪,以及宁波美康和北京九强生化试剂进行抗干扰试验。方法试验以血红蛋白作为溶血干扰物质,甘油三月桂酸酯作为脂血干扰物质,胆红素作为黄疸干扰物质,分别配制干扰梯度浓度,加入混合血清进行仪器测试,基准组加入等量生理盐水,分析标本不同溶血、脂血、黄疸水平对各项生化指标的影响。结果溶血影响下,心肌酶类项目的血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)明显上升,干扰物在1g/L时分别有23.20%、16.96%、26.90%的正偏差,已超过可允许范围,其余项目影响在可允许范围内。脂血影响下,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TB)、直接胆红素(DB)轻度下降,总蛋白(TP)、CK、CK-MB轻度上升,干扰物在12mmol/L时偏差超过可允许范围,其余项目影响在可允许范围内。黄疸影响下,血清CK-MB、总胆固醇(TC)、三酰甘油(TG)轻度下降,干扰物在600μmol/L时偏差超过可允许范围,其余项目影响在可允许范围内。结论该研究检测体系的抗干扰能力符合要求,可为临床该3类标本的判断和处理提供依据。

Objective Based on the current test system Hitachi HITACHI 7180 biochemical analyzer,biochemical reagents provide by Ningbo Medicalsystem Biotechnology and Beijing Strong Biotechnologies.Our conduct anti-interference experiments.Methods The hemoglobin was used as the hemolysis interfering substance,glycerol trilaurate as the lipid interference substance,the bilirubin as the jaundice interfering substance.The interfering gradient liquid was prepared separately and added to the mixed serum for testing.The reference group was given the same amount of normal saline.And test it to study the extent of the impact on various biochemical indicators in the serum samples of different hemolysis,lipids,jaundice.Results Under the influence of hemolysis,the CK,CK-Mb and LDH increased significantly,while the rest of the items had less influence.The positive deviation were 23.20%,16.96%,26.90% respectivly while the interreference were 1 g/L,which was out of limited.The deviation in other was allowed.Under the influence of lipids the levels of total bilirubin,direct bilirubin,ALT and AST decreased slightly;TP,CK,CK-Mb slightly increased.Under the influence of jaundice,CKMB,TC,TG slight decline,the rest of the project less affected.Conclusion The antiinterference ability of the test system meets the requirements.The clinical examination of the results of these three types of samples can be judged and processed according to the relevant data of this research.