枸杞醋镇静催眠作用的实验研究

Experimental Study on Sedative and Hypnotic Effects of Whortleberry Vinegar

目的探讨枸杞醋的镇静催眠作用,为开发具有辅助改善睡眠功能的保健食品提供依据.方法将雄性ICR小鼠随机分成5组,即空白对照组(灌胃蒸馏水)、地西泮组(灌胃地西泮)、枸杞醋低剂量组(灌胃枸杞醋25%)、枸杞醋中剂量组(灌胃枸杞醋50%)、枸杞醋高剂量组(灌胃枸杞醋100%),各组小鼠灌胃1次/d,连续7 d后进行行为学实验.自主活动仪观察枸杞醋对小鼠活动及站立次数的影响;阈下/阈剂量戊巴比妥协同睡眠实验观察枸杞醋对小鼠睡眠只数、睡眠潜伏期及睡眠时间的影响;采用酶联免疫吸附法(Enzyme-linked Immunodef Ic ient assay,ELISA)检测小鼠脑组织中γ-氨基丁酸(γ-aminobutyricac Id,GABA)和谷氨酸(Gluteus,GLU)含量.结果与空白组比较,枸杞醋50%,100%剂量组小鼠自主活动次数及站立次数明显减少(P<0.01,P<0.05);枸杞醋各剂量组小鼠睡眠只数明显增加,枸杞醋100%剂量组睡眠潜伏期被明显缩短(P<0.05),睡眠时间明显延长;枸杞醋50%,100%剂量组小鼠脑内GABA含量明显升高,GLU含量明显降低,GABA/GLU比值明显增加(P<0.05,P<0.01).结论枸杞醋在实验中对小鼠具有镇静催眠的作用,其机制可能与其调节脑组织中GABA及GLU含量有关.

Objective To study the sedative and hypnotic effects and its mechanism of Chinese Whortleberry vinegar and to provide a basis for the development of health food products with auxiliary sleep function. Method Male ICR mice were randomly divided into five groups： blank control group（ distilled water）,diazepam group（ diazepam administration）,low dose group of Whortleberry vinegar（ 25% of Chinese Whortleberry vinegar）,middle dose group of Whortleberry vinegar（ 50% of Chinese Whortleberry vinegar）,high dose group of Whortleberry vinegar（ 100% of Chinese Whortleberry vinegar）. The mice were intragastrically administered with the corresponding agents for 7 days,once daily. The locomotor activity of mice was observed with a multifunctional locomotor activity recorder,and the hypnotic effects were observed in mice treated with the subthreshold dose and threshold dose of pentobarbital sodium. The levels of γ-aminobutyric acid（ GABA） and glutamate（ Glu） in the brain of mice were detected by enzyme-linked immunosorbent assay（ ELISA）. Results Compared with the blank group,the numbers of autonomic activities and standing times of mice in the Whortleberry vinegar 50%,100% dose groups significantly decreased（ P〈0.01,P〈0.05）,the numbers of sleeping mice in the three doses of Whortleberry vinegar groups were higher,and the sleep latency was significantly shorter in the 100% dose group（ P〈0.05）,meanwhile the sleep duration was obviously longer in the Whortleberry vinegar（ 100%） group treated with the threshold dose of pentobarbital sodium. Whortleberry vinegar（ 50%,100%） could significantly increase the level of GABA and decrease the level of Glu,and also increase their ratios of GABA/Gl U in the brain tissues（ P〈0.05,P〈0.01）. Conclusion Whortleberry vinegar has obvious sedative and hypnotic effects in mice,which may be related to its regulating the content of GABA and Gl U in the brain tissues.