转Bt基因抗虫玉米HGK60的农艺性状分析

Agronomic Traits Analysis of Transgenic Btcry1Ah Maize HGK60 Line

为了确定转Bt cry1Ah抗虫玉米HGK60的自交系及其杂交后代外源基因的遗传表达稳定性和农艺性状,通过实时荧光定量PCR和ELISA分析外源基因的遗传表达稳定性,通过室内外生测和田间性状考量分析农艺性状。荧光定量PCR结果表明Bt cry1Ah基因在玉米的不同组织中可以正常转录,但RNA表达水平存在一定的差异;ELISA结果表明在转基因植株的不同发育时期、不同器官中Cry1Ah的蛋白表达量顺序:雄穗>叶片>苞叶>籽粒>花丝>穗轴。两地连续三代的田间及室内抗虫性检测结果表明HGK60抗虫玉米对亚洲玉米螟均表现出很好的抗性。性状考量结果表明HGK60抗虫玉米与受体材料对照比较,种子发芽率、生育期、穗行数、穗长、千粒重等农艺性状均无显著差异。通过多年多点田间试验和分子检测结果证明HGK60转基因抗虫玉米中外源基因稳定的遗传和表达,对亚洲玉米螟有很好的抗性,农艺性状与对照材料无显著差异。HGK60转基因抗虫玉米对亚洲玉米螟的良好抗性使其具有很好的产业化应用前景。

To determine the genetic expression stability and agronomic traits of the inbred lines of Bt cry1 Ah-resistant maize HGK60 and its hybrid progenies,we analyzed the genetic expression stability of the exogenous genes by fluorescence quantitative PCR and ELISA,both bioassay indoor and outdoor. Analysis of agronomic traits were carried out by bioassay and agronomic traits considerations. The result offluorescent quantitative RT-PCR showed that Bt cry1 Ah could be transcribed normally in different plant tissues,and the expression level ofRNA was different. The results of ELISA showed that the protein expression of Cry1 Ah in different organs at different developmental stages ：tassel leaf husk grain filament cob. Through two locations and three consecutive generations of bioassay indoor and outdoor,theHGK60 line showed high resistant to Asian corn borer. Compared with the recipient inbred and hybrid lines,seed germination rate,growthperiod,ear number,spike length,thousand grains weight and other agronomic traits considerations indicated are not significantly different.Through experiments and molecular tests tracking over many years,the genetic and expression stability of the exogenous genes in HGK60 transgenic maize has been proven to be very resistant to Asian corn borer,and there is no significant difference between agronomic traits andcontrol materials. The good resistance of HGK60 transgenic insect-resistant corn to Asian corn borer proved that HGK60 transgenic insectresistant maize has good commercial application prospect.