ZMYND11在多形型胶质母细胞瘤中的表达及意义

Expression and Clinical Significance of ZMYND11 in Glioblastoma Multiforme

目的探讨ZMYND11在多形性胶质母细胞瘤(GBM)中的表达及意义。方法收集河北医科大学第二医院GBM患者术中肿瘤标本20例(肿瘤组),重度脑外伤患者正常脑组织标本20例(对照组),对上述标本进行Western blot及qRT-PCR实验,检测并比较两组ZMYND11的表达;利用ZMYND11过表达的慢病毒转染GBM的细胞系U87细胞使其ZMYND11过表达,通过CCK、Transwell及流式细胞分析检测ZMYND11对U87细胞在增殖、侵袭及凋亡方面的作用;将ZMYND11过表达的U87细胞接种至裸鼠内进行体内试验。结果肿瘤组中ZMYND11的表达量明显低于对照组(P<0.001);ZMYND11过表达可明显抑制U87细胞的增殖及侵袭并促进其凋亡,体内实验显示ZMYND11可明显抑制肿瘤的生长。结论 ZMYND11可抑制GBM的发生与发展。

Objective To investigate the expression and clinical significance of ZMYND11 in glioblastoma multiforme（GBM）. Methods The tumor samples（tumor group） and non-neoplasm brain tissue（control group） were collected intraoperatively from 20 cases of GBM and 20 patients with severe brain injury in the Second Hospital of Hebei Medical University. The difference of ZMYND11 expression between two groups was determined using qRT-PCR and Western blot. U87 cells with upregulated ZMYND11 expression was constructed using transfection of ZMYND11-overexpression lentivirus. CCK, Transwell assay and flow cytometry were performed to identify the role of ZMYND11 in the proliferation, invasion and apoptosis of U87 cells. U87 cells with ZMYND11 overexpression were inoculated into nude mice for in vivo experiment. Results The expression levels of ZMYND11 mRNA and protein were lower in GBM tissues than those in non-neoplasm brain tissues（P〈0.001）. In vitro, ZMYND11 inhibited effectively the proliferation and invasion, as well as promoted the apoptosis of U87 cells. In vivo, ZMYND11 suppressed remarkably the growth of graft tumor. Conclusion ZMYND11 inhibits the development and progression of GBM.