钨酸钠对成熟期烤烟氮代谢、氧化胁迫和烟碱含量的影响

Effects of Na_2WO_4 on nitrogen metabolism, oxidative stress and nicotine content in cured tobacco leaves at maturity stage

为解决烟叶后期贪青晚熟所造成的问题,本研究以K326为研究对象,针对生长过旺的烟株喷施不同浓度的钨酸钠,研究了其对烟叶根系活力、氮代谢、抗氧化系统及烟碱含量的影响。结果表明:喷施钨酸钠不同程度地减弱了烟株的根系活力、硝酸还原酶(NR)活性、谷氨酰胺合成酶(GS)活性、丙二醛(MDA)含量,并与钨酸钠浓度呈显著负相关;增加了超氧化物歧化酶(SOD)、过氧化物酶(POD)等抗氧化酶活性,与钨酸钠的浓度呈显著正相关;同时降低了烟碱的含量。综合来看,以喷施1.0 mmol/L的钨酸钠能够有效抑制烟叶的氮代谢,促进烟叶成熟落黄,提高烟叶抗氧化胁迫的能力,同时降低烟叶烟碱含量。

In order to solve the problem caused by the late maturing tobacco, the effects of different concentrations of Na_2WO_4 sprayed in overgrown tobacco plants on the tobacco root activity,nitrogen metabolism,antioxidant system,nicotine content were studied using K326 as the research object. The results indicated that the root activity,nitrate reductase（NR） activity,glutamine synthetase（GS）,malondialdehyde（MDA） content were negatively correlated with the concentration of Na_2WO_4. The activities of invertase（ Inv）,SOD,and POD were increased,which were positively correlated with the concentration of Na_2WO_4. At the same time,the content of nicotine was decreased. In general,spraying 1. 0 mmol/L of Na_2WO_4 could effectively inhibit the nitrogen metabolism of tobacco leaves,and promote tobacco leaf turning yellow,improve the ability of tobacco resistance to oxidative stress,and reduce the nicotine content of tobacco leaves.