辣椒bZIP家族基因的鉴定与表达分析

Genome Wide Identification and Expression Analysis of the bZIP Gene Family in Pepper

利用生物信息学的方法对辣椒bZIP家族基因进行全面鉴定与进化分析,并在此基础上对基因染色体定位、蛋白质保守基序和基因结构等进行分析,并通过qRT-PCR方法检测基因的组织表达模式和在ABA胁迫下的应答等。结果表明,从辣椒基因组中共鉴定出54个bZIP家族基因,这些基因分散分布在辣椒的11条染色体上;系统进化分析表明辣椒bZIP基因家族可以分为10组,每组所含有的基因数不等;该家族成员每个基因含有0~11个内含子;bZIP家族基因具有不同的表达模式;外源激素ABA处理可以明显抑制或者激活该家族基因成员的表达。

In this study,we systematically identified genes from the genome of pepper and elucidated their phylogenetic relationships by bioinformatics. Based on the pepper whole genome sequence,CabZIP gene family chromosome location,conserved motif,gene structure,tissue-specific transcription patterns and response to ABA stress were analyzed. The results showed that a total of 54 CabZIP genes were identified and they were distributed on 11 chromosomes. The pepper bZIP gene family were divided into 10 groups and each of group included a range of genes. This gene family contained 0–11 introns. The CabZIP gene family exhibited distinct expression pattern in different tissues. The CabZIP gene could be repressed or activated by ABA treatment. Those results will provide a foundation for further functional research on CabZIP genes in pepper.