海地瓜多肽体外降糖活性评价

Evaluation of hypoglycemic activity in vitro for polypeptides from Acaudina molpadioides

目的:运用多种体外活性筛选方法评价海地瓜多肽的降糖活性,为海地瓜多肽进一步开发利用提供支持。方法:通过α-淀粉酶抑制剂筛选方法、DPP4抑制剂筛选方法及测定高糖损伤后胰岛细胞分泌胰岛素含量等多种体外降糖评价方法测定了海地瓜多肽的降糖活性。结果:多种体外降糖活性评价结果显示,海地瓜多肽可有效抑制α-淀粉酶活性、DPP4活性,同时可显著提升高糖损伤胰岛β细胞的胰岛素分泌量。结论:海地瓜多肽具有一定的降糖活性,可为后期海地瓜多肽的研发提供一定的理论支持。

Objective： To evaluate the hypoglycemic activity for polypeptides from Acaudina molpadioides by a variety of active screening methods in vitro, and to provide support for the further development and utilization of polypeptides from Acaudina molpadioides. Methods： The hypoglycemic activity of polypeptides from Acaudina molpadioides were determined by a variety of hypoglycemic evaluation methods in vitro, such as screening of a-amylase inhibitor, screening of DPP4 inhibitor and determination of insulin secretion in islet cells after high glucose injury. Results： The evaluation results showed that the polypeptides from Acaudina molpadioides can inhibit the activity of a-amylase and DPP4 effectively, and can enhance the high glucose-induced pancreatic β-cell insulin secretion significantly. Conclusion： The polypeptides from Acaudina molpadioides has certain hypoglycemic activity, which may provide theoretical support for the further development of the polypeptides from Acaudina molpadioides.