冷鲜鸡胸肉中2种优势腐败菌的分离鉴定

Identification of two dominant spoilage bacteria isolated from chilled chicken breast

以冷鲜鸡胸肉为对象,研究在4℃冷藏条件下腐败菌菌相变化,并对其优势腐败菌进行分离鉴定。4℃下冷藏开始时,冷鲜鸡胸肉中假单胞菌属(Pseudomonas)和环丝菌属(Brochothrix)菌落总数之和仅占细菌总数的24%。随着冷藏时间的延长,两菌属迅速增长繁殖,于第2天成为优势腐败菌群,并于第(2~8)天持续占据优势。对优势菌落进一步分离纯化得到两株优势腐败菌CM1、CM2。通过传统微生物生理生化鉴定和Biolog GenⅢ表型测试对2菌株进行初步鉴定,并进一步结合16S r DNA序列分析建立系统发育树,确定CM1菌株与Pseudomonas deceptionensis、CM2菌株与Brochothrix thermosphacta亲缘关系最近。该研究结果可为冷鲜鸡胸肉的贮藏保鲜及抑菌研究提供理论依据。

In order to investigate the change of bacterial flora during cold storage and the identification of dominant spoilage bacteria in chilled chicken, the chilled chicken breast was selected as raw material. At the beginning of cold storage within chilled chicken breast, Pseudomonas and Brochothrix made up only 24% of the total bacteria, with the extension of cold storage time, the two spoilage bacterial propagate rapidly and become the dominant spoilage bacteria on the second day. In addition, they continued to dominate in （2-8）d. Two dominant spoilage bacteria, CM1 and CM2, were further isolated and purified from the two dominant spoilage colonies. The microorganisms were isolated and identified by the traditional physio-biochemical tests and Biolog Gen Ⅲ phenotype analysis combined with 16S rDNA sequencing method constructing phylogenetic tree. The genetic relationships between CM1 and Pseudomonas deceptionensis as well as CM2 and Brochothrix thermosphacta were confirmed to be the most similar, The results would provide a theoretical basis and technical support for the preservation of chilled chicken breast during cold storage.