NAC修饰的CdS测定盐酸帕罗西汀片剂含量的研究

Determination of Paroxetine Hydrochloride Tablet by CdS Quantum Dots Modified with N-acetylcysteine

以N-乙酰半胱氨酸(NAC)为修饰剂,简便,无毒,快速合成了CdS量子点,并基于盐酸帕罗西汀对该量子点的荧光猝灭作用,建立了盐酸帕罗西汀片剂的含量测定方法。结果显示,在pH=7.22,Tris-HCl缓冲液浓度为0.06 mol/L,量子点浓度为0.35 mmol/L,反应25 min的最佳条件下,该系统荧光强度变化值与盐酸帕罗西汀在(0.0033~0.1810 mg/m L)范围内线性关系良好,ΔF=682.06C+19.115为线性回归方程,检测限为0.32μg/m L。结果满意。

A new protocol for the determination of paroxetine hydrochloride was presented based on the fluorescence quenching of high quality CdS quantum dots successfully synthesized with N-acetylcysteine as modifiers. The results showed that when the concentration of CdS was 0. 35 mmol/L,the concentration of Tris-HCl buffer solution was0. 06 mol/L and the reacting time was 25 minutes at pH = 7. 22,the value of fluorescence intensity changes was linearly proportional to the concentration of paroxetine hydrochloride in the range of 0. 0033 - 0. 1810 mg/mL. The linear equation was ΔF = 682. 06 C ＋ 19. 115 and the detection limit was 0. 32 μg/m L. The result of the determination of marketed paroxetine hydrochloride tablets was satisfying.