慢性乙型肝炎患者共感染EBV时外周血单个核细胞IL-21 mRNA的表达水平

Expression level of PBMC IL-21 mRNA in peripheral blood of chronic hepatitis B patients with CO infection of EBV

目的探讨慢性乙型肝炎患者共感染EB病毒(EBV)时外周血单个核细胞(PBMC)白细胞介素-21(IL-21)mRNA的表达水平情况。方法收集20例乙型肝炎病毒(HBV)、EBV共感染者(病例组),20例单纯HBV感染者(对照组)和20例体检健康者(健康组)血样及临床资料,Real-time PCR分析外周血PBMC IL-21mRNA表达。结果病例组IL-21 mRNA表达水平明显高于对照组和健康组(P<0.05),对照组IL-21mRNA表达水平明显高于健康组(P<0.05);病例组患者均发低热,对照组、健康组体温正常;病例组ALT、AST明显高于对照组和健康组(P<0.05),对照组ALT、AST亦明显高于健康组(P<0.05)。结论 IL-21在HBV感染者中表达上调,HBV、EBV共感染者更甚,其可能是机体控制病毒复制的机制之一。

Objective To investigate the expression of IL-21 mRNA in peripheral blood mononuclear cells （PBMC） of patients with chronic hepatitis B co-infected with EBV. Methods Blood samples and clinical data of 20 HBV,EBV co-infected patients （case group）,20 HBV-infected patients （control group） and 20 healthy subjects （healthy group） were collected.Real-time PCR analysis of peripheral blood PBMC IL -21 mRNA expression. Results The expression of IL-21 mRNA in the case group was significantly higher than that in the control group and the healthy group （ P 〈0.05）.The expression of IL-21 mRNA in the control group was significantly higher than that in the healthy group （ P 〈0.05）.The control group and healthy group had normal body temperature;ALT and AST in the case group were significantly higher than those in the control group and healthy group （ P 〈0.05）,and ALT and AST in the control group were significantly higher than those in the healthy group （ P 〈0.05）. Conclusion IL-21 is up-regulated in HBV-infected patients,and HBV and EBV co-infected patients are more likely,which may be one of the mechanisms by which the body controls viral replication.