IgA肾病患者肾脏组织中差异细胞因子的探索研究

Differential proteins screening: cytokines in renal tissues from patients with IgA nephropathy

目的筛选IgA肾病(IgAN)患者肾脏组织中的差异蛋白质-细胞因子,进一步探讨IgAN的发病机制。方法入选年龄18~60岁,解放军总医院肾脏病科住院肾活检确诊为IgAN患者10例;泌尿外科行肾癌肾脏切除的患者10例,取癌旁6 cm以外正常肾脏组织作为对照。肾脏组织利用L-493细胞因子抗体芯片进行检测,筛选差异蛋白质,并对差异蛋白质进行基因本体论(GO)分析。以IL-33(白介素-33)利用酶联免疫吸附试验(ELISA)进行验证。采用IBM SPSS Statistics 19.0软件对结果进行统计分析,利用T检验对差异蛋白质进行筛选,P<0.05,并且差异蛋白质差异倍数>1.4认为是差异蛋白质。结果 L-493细胞因子抗体芯片检测,在20例患者中(IgAN组10例,对照组10例),发现了25种差异蛋白质,GO分析结果表明:这些蛋白质主要与应激、细胞粘附、蛋白质代谢、信号转导相关。ELISA验证结果显示:IgAN患者肾组织中IL-33表达水平显著高于对照组。结论 IgAN患者肾脏组织中差异表达蛋白质直接或间接调控着炎症、细胞增殖和凋亡及纤维化过程。IL-33可能与IgAN发病机制有关。

Objective Screening the differential proteins of cytokines in renal tissues from patients with IgA nephropathy（ IgAN） in order to elucidate the pathogenesis of IgAN. Methods 10 patients,18 to60 years old,diagnosed as IgAN according to the renal biopsies in our hospital,were selected. 10 cases of renal cell carcinoma in department of urology of our hospital,aged 18 to 60 years,were chosen. Normal renal tissues 6 cm away from the renal cancer were taken as controls. The L-493 cytokine antibody microarray was used to screen the renal tissues for differentially-expressed proteins which were subsequently analyzed with the gene ontology（ GO） method. Then interleukin-33（ IL-33） was used for validation in enzyme linked immune sorbent assay（ ELISA）. Results A total of 25 differentially-expressed proteins were detected. The GO analysis showed that these proteins were related to stress,cell adhesion,protein metabolism,and signal transduction. And the ELISA results showed that the expression of IL-33 in the renal tissues of patients with IgAN was significantly higher than that in the control group. Conclusions The differentially-expressed proteins in the renal tissues of IgAN patients may directly or indirectly regulate inflammation, cell proliferation,apoptosis,and fibrosis. IL-33 may be involved in the pathogenesis of IgAN.