纯钛表面经微弧氧化处理后对成纤维细胞生长的影响研究

Influence of pure titanium surface treated by microarc oxidation on the growth of fibroblasts

目的比较纯钛经不同表面处理后对成纤维细胞生长的影响。方法将钛片随机分为微弧氧化组、酸蚀喷砂组、光滑组并分别进行表面处理，对处理后的试件表面进行理化分析。将小鼠成纤维细胞接种于经不同处理的钛片表面，采用噻唑蓝MTT法检测成纤维细胞在不同钛片表面的黏附和增殖情况。结果显示细胞在经微弧氧化处理的试件表面的黏附、增殖情况均好于其他两组。结论经微弧氧化技术处理后的纯钛有利于与牙周软组织结合形成生物学封闭。

Objective To evaluate the effects of adhesion and proliferation of fibroblasts cultured in pure titanium surfaces having undergone different kinds of surface treatment. Methods Test pieces of titanium plate were ground with water resistant sand paper and polished and then randomly divided into 3 groups： smooth group without further processing, sandblasting and large-grits etching （SLA） group underegoing vertical jet with A1203 particles ,and microarc oxidation group processed by pulse DC power supply. Five pieces from each group were put in 24 hole culture plate into which single cell suspension of mouse fibroblasts was inoculated and cultured for 1, 2, and 4 hours. Microplate reader was used to test the absorbanee so as to observe the adhesion of the fibroblasts on the surface of test pieces. Another 5 pieces from each group were put in 24 hole culture plate into which single cell suspension of mouse fibroblasts was inoculated and cultured for 1, 3, and 5 days.MTT method was used to detect the absorbance so as to observe the proliferation of the fibroblasts on the surface of test pieces. Result The roughness degree of the mieroarc oxidation group was significantly higher than that of the mieroarc oxidation group, and the Roughness degree of the mieroare oxidation group was in turn significantly higher than that of the smoth group （all P〈0.05）. The contact angle of the smoth group was signififcantly bigger than that of the SLA group, and that of the SLA group was in turn significantly bigger than that of the microarc oxidation group （all P〈0.05）. The degrees of absorbance of the test piece surface at the time points of 1, 2, and 4 hours of the 2 processed groups were all significantly higher that those of the smotth group （all/）〈0.05）, and the absorbance degrees at the time points of 1, 2, and 4 hours of the SLA group were significantly higher those of the microarct oxidation group （all P〈0.05）. Conclusion The adhesion and proliferation of the fibroblasts on the surface of the micro arc oxidation specimens are better than those of the other two groups. Microarc oxidation surface is good for soft tissue attachment on implant abutment.