MCM7和P27在肝细胞癌中的表达及临床意义

Expression and clinical significance of MCM7 and P27 in hepatocellular carcinoma

目的探讨MCM7和P27蛋白在肝细胞癌组织中的表达及与肝细胞癌发生、发展和临床病理因素的关系。方法选取2013年1月至2015年8月间邯郸市中心医院收治的60例原发性肝癌患者的肝细胞癌组织和癌旁组织标本,另选取20例正常肝组织为对照。采用免疫组织化学(IHC)S-P法检测3种肝组织中MCM7和P27蛋白的表达情况,结合患者病理指标进行分析。结果MCM7在肝细胞癌组织中的阳性表达率为73.3%(44例),在癌旁硬化肝组织和正常肝组织中未见明显表达,差异均有统计学意义(均P<0.05)。肝细胞癌中P27蛋白的阳性率为43.3%(26例),低于癌旁组织的83.3%(50例)和正常肝组织的85.0%(17例),差异均有统计学意义(均P<0.01)。Spearman等级相关分析显示,肝细胞癌中MCM7蛋白与P27蛋白的表达呈负相关(P<0.01)。结论MCM7蛋白高表达和P27蛋白低表达在肝细胞癌的发生中可能起到重要作用。

Objective To investigate the expression of MCM7 and P27 proteins in hepatocellular carcinoma tissues,and the relationship between the expression of MCM7 and P27 proteins and the occurrence and development and clinicopathological factors of hepatocellular carcinoma（HCC）.Methods Cancer tissues and pericancer tissues from 60 patients with primary HCC who were treated at Handan Central Hospital between January 2013 and August 2015 were selected.Another 20 normal liver tissues were selected as the controls.Immunohisto Chemistry（IHC） S-P was used to detect the expression of MCM7 and P27 protein in hepatocellular carcinoma tissues,adjacent liver tissues and normal liver tissues,and combined with the clinical pathological indexes,statistical analysis was performed.Results The positive expression rate of MCM7 in HCC tissues was 73.3%（44 patients）.MCM7 protein was not significantly expressed in the pericancer tissues and normal liver tissues（all P〈0.01）.The positive rate of P27 protein in hepatocellular carcinoma was 43.3%（26 patients）,which was significantly lower than 83.3%（50 patients） of the pericancer tissues and 85%（17 patients） of the normal liver tissues（all P〈0.01）..Spearman rank correlation analysis showed that the expression of MCM7 protein was negatively correlated with the expression of P27 protein in hepatocellular carcinoma（P〈0.01）.Conclusion The high expression of MCM7 protein and the low expression of P27 protein play an important role in the incidence of hepatocellular carcinoma,which may provide a new target for future research on this disease.