介质液液萃取-气相色谱/串联质谱法测定血液中4种吩噻嗪类药物

The simultaneous determination of 4 phenothiazines in blood by supported liquid extraction and GC/MS/MS

目的研究建立介质液液萃取-气相色谱/串联质谱分析血液中吩噻嗪类药物的方法。方法采用介质液液萃取技术处理血液样品,乙醚进行洗脱,然后用气相色谱/串联质谱仪测定。结果 4种吩噻嗪类药物的检测限在1.0 ng/mL^3.3 ng/mL之间,线性范围25 ng/mL或50 ng/mL^1000 ng/mL,以空白血液样品为基体进行回收试验测得回收率为73.8 ng/mL^104.1 ng/mL,测定值的相对标准偏差(n=5)在4.7%~10.2%之间。结论 SLE-GC/MS/MS检测法可用于血液中吩噻嗪类药物的检测分析且该方法具有良好的灵敏度、回收率、精密度及重现性。

Objective To establish the analytical method of phenothiazines in blood by SLE-GC/MS/MS.Methods Samples were extracted with supported liquid extraction columns and eluted by ether.The collected solutions were analyzed by GC-MS/MS.Results For all analytes,LODs were 1.0 ng/mL^3.3 ng/mL,linear curve covered the range 25 ng/mL or 50 ng/mL^1000 ng/mL,recoveries were 73.8 ng/mL^104.1 ng/mL and the relative standard deviations were in the range of 4.7%~10.2%.Conclusion It had showed that a simultaneous analysis of phenothiazines in blood by SLE-GC/MS/MS was established with a reasonable LOQ,linear range,recovery and high sensitivity,which can be used for determination of phenothiazines in blood.