摘要
为了研究不同因素对紫花苜蓿萌发的影响,试验采用正交试验设计,测定了浸种药剂（纯化水、0.1%水杨酸、5%聚乙二醇、2%KNO3、0.5%Na OH）、浸种时间（0,1,2,3,4 h）、Na2CO3浓度（0,5,15,25,35 mmol/L）三因素条件下的新疆大叶紫花苜蓿种子发芽率、发芽势和发芽指数;又采用单因素试验设计,研究了单因素Na2CO3浓度（0,5,10,15,20,25,30,35 mmol/L 8个浓度）下种子的发芽率、发芽势和发芽指数。结果表明：浸种药剂、浸种时间、Na2CO3浓度对发芽率和发芽势皆有显著影响（P〈0.05）,Na2CO3浓度对发芽指数有显著影响（P〈0.05）,而浸种药剂、浸种时间对发芽指数无显著影响（P〉0.05）,且对发芽率、发芽势和发芽指数的影响次序皆为Na2CO3浓度〉浸种时间〉浸种药剂;发芽率、发芽势、发芽指数皆随Na2CO3浓度的升高而呈下降趋势;新疆大叶紫花苜蓿种子对Na2CO3的耐盐半致死浓度范围为20-25 mmol/L,致死浓度范围为30-35 mmol/L。说明新疆大叶紫花苜蓿种子的最适萌发条件为无需浸种、不加Na2CO3。
The aim of the present study was to investigate theeffects of different factors on Medicago sativa. According to orthogonal experimental design,germinate rate,germinate energy,germinate index of Medicago sativa cv. Xinjiang Daye under seed-soaking agent（ distilled water,0. 1% salicylic acid,5% PEG,2% KNO3,0. 5% Na OH）,seed-soaking time（ 0,1,2,3,4 h） and Na2CO3 concentration（ 0,5,15,25,35 mmol/L） were determined. Furthermore,germinate rate,germinate energy,germinate index of M. sativa cv. Xinjiang Daye were determined by single-factor experimental design in eight Na2CO3 concentrations（ 0,5,10,15,20,25,30,35 mmol/L）. The results showed that seed germination rate and germination energy were significantly affected by seed-soaking agent,seed-soaking time and Na2CO3 concentration（ P <0. 05）. Germination index was significantly affected by Na2CO3 concentration（ P<0. 05）,but not by seed-soaking reagent and seed-soaking time（ P>0. 05）.The order of effect on germination rate,germinate energy and germination index was Na2CO3 concentration>seed-soaking time>seed-soaking reagent.The germination rate,germination energy and germination index decreased with the increase of Na2CO3 concentration.The half-lethal Na2CO3 concentration of M. sativa cv. Xinjiang Daye seed was 20-25 mmol/L,and the lethal Na2CO3 concentration was 30-35 mmol/L. The results indicated that the optimum germination condition of M. sativa cv. Xinjiang Daye seed was no soaking and no Na2CO3.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2018年第15期151-155,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31772669
31702178)
国家重点研发计划项目(2016YFD501005-3)
吉林省省校共建计划专项(SXGJXX2017-4)
吉林省科技发展计划项目(20170307018NY)
吉林省自然科学基金项目(20150101089JC)
吉林省高教学会高教科研课题2017年度立项课题(JGJX2017D6
JGJX2017D5)
关键词
新疆大叶紫花苜蓿
发芽率
浸种药剂
NA2CO3胁迫
正交试验设计
种子萌发
耐盐半致死浓度
耐盐致死浓度
Medicago sativa cv Xinjiang Daye
germination rate
seed soaking agent
Na2CO3 stress
orthogonal experimental design
seed ger mination
half-lethal concentration
lethal concentration
