八味沉香散对局灶性脑缺血大鼠VCAM-1表达的影响

Effects of Bawei Chenxiang powder on expression of VCAM-1 in focal cerebral ischemia in rats

目的:探讨八味沉香散对局灶性脑缺血大鼠脑组织中血管细胞黏附分子-1(VCAM-1)表达的影响。方法:雄性SD大鼠360只,随机分为假手术组、模型组、八味沉香散大剂量组(0.8 g/kg)、八味沉香散中剂量组(0.4 g/kg)和八味沉香散小剂量组(0.2 g/kg)。每组随机分为1 h组、6 h组、12 h组、24 h组、72 h组、120 h组6个时相组,每一时相12只大鼠。采用改良Zea Longa线栓法制备大脑中动脉(MCAO)栓塞模型,实时荧光定量(real-time PCR)检测八味沉香散干预对局灶性脑缺血大鼠不同时相VCAM-1在mRNA水平表达的影响。结果:与假手术组比较,模型组神经功能评分显著升高,且模型评分均>2分,证明模型制作成功。VCAM-1在脑缺血损伤1 h后开始上升,于24 h达到高峰,之后逐渐下降(P<0.05);八味沉香散各剂量组VCAM-1表达较模型组降低(P<0.05)。结论:八味沉香散对局灶性脑缺血损伤具有明显的保护作用,通过降低血管细胞黏附分子-1对局灶性脑缺血大鼠的炎性反应,减轻脑缺血后大鼠的神经元损伤,对缺血性脑损伤起到保护作用。

Objective ： To investigate the effect of Bawei Chenxiang powder on the expression of vascular cell adhesion molecule - 1 （ VCAM - 1 ） in the rat brain after focal cerebral ischelnia. Methods：360 male SD rats were randomly divided into sham operation group, model group, high - dose group of Bawei Chenxiang powder （0.8 g/kg）, middle - dose group of Bawei Chenxiang powder （0.4 g/kg） and small - dose group of Ba- wei Chenxiang powder（0.2 g/kg）. Each group was randomly divided into l h group, 6h group, 12h group, 24 h group, 72 h group, 120 h group. There were 12 rats in each group. The MCAO elnbolization model was prepared by the modified Zea Longa method. Real - time fluorescence quan- titative real - time PCR was used to detect the expression of VCAM - 1 in rats with focal cerebral ischelnia after interention of Bawei Chenxiang Powder. The effect of Bawei Chen Xiang Powder on the expression of VCAM - 1 mRNA in brain tissue of rats with cerebral ischelnia at different time points was analyzed. Results ： Compared with the sham - operated group, the neurological function scores of the model group were significantly higher, and the model scores were 2 and more points, which proved the success of the model. VCAM - 1 began to increase 1 hour after cerebral is- chemic injury, peaked at 24 hours, then decreased gradually （ P 〈 0.05） ; the expression of VCAM - 1 in Bawei Chenxiang powder was signifi- cantly lower in each dose group than in the model group （ P 〈 0.05 ）. Conclusion ：Bawei Chenxiang powder has a protective effect on focal cere- bral ischelnic injury. It can reduce the inflanunatory response of focal cerebral ischelnia in rats by reducing vascular cell adhesion molecule - 1, and alleviate neuronal damage in rats？ after cerebral ischelnia, protecting the ischelnic brain damage.