结肠癌中相关组蛋白乙酰化修饰位点的机制探讨

Mechanism of histone acetylation modification site in colon cancer

目的观察结肠癌细胞株SW480及结肠癌组织中组蛋白乙酰化修饰的相关位点的变化情况。方法实时无标记细胞分析仪(RTCA xCELLigence)动态观察人正常结肠上皮细胞株NCM460及人结肠癌细胞株SW480的增殖情况;Western blot检测正常结肠细胞株及结肠癌细胞株的组蛋白acH3K9、acH3K14、acH3K18、acH3K27修饰的情况及同一肠癌患者手术后切除的肠段肿瘤部位(结肠癌组织)和非肿瘤部位(正常结肠上皮组织)组蛋白acH3K9、acH3K14、acH3K18、acH3K27修饰的情况。结果结肠癌细胞株SW480增殖速度明显大于正常结肠上皮细胞株的增殖;相同培养状态下,在72h后人结肠癌上皮细胞株增殖速度明显大于正常结肠上皮细胞株的增殖;与NCM460相比,SW480细胞组蛋白acH3K9、acH3K14、acH3K18、acH3K27修饰下调(P<0.01)。结论结肠癌的发生过程中,在表观遗传学方面,可能也有一定的关联,而组蛋白乙酰化修饰下调可能是引起结肠癌发生发展机制的又一条通路。

Objective To observe the changes of histone acetylation in colon cancer cell line SW480 and colon cancer tissue,to explore the possible mechanism of histone acetylation in the development of disease.Methods Realtime unmarked cell analyzer（RTCA xCELLigence）was used to observe the proliferation of normal colonic epithelialcell line NCM460 and human colon cancer cell line SW480.Western blot（WB）test normalcolonic cell lines and colon cancer cell lines of histone acH3K9,acH3K14,acH3K18,acH3K27 modified conditions and same colorectal cancer patients after surgery resection of the intestine tumor site（colon tissue）and the tumor location（normal colon epithelial tissue）histone acH3K9,acH3K14,acH3K18,acH3K27 modifications.Results SW480 of colon cancer cell lines obviously proliferated faster than normal colonic epithelial cell lines.In the same culture,the proliferation of the cell lines was faster than that of normal colonic epithelial cell lines after 72 h.Compared with NCM460,SW480 cell protein acH3K9,acH3K14,acH3K18,acH3K27 modified（P0.01）.ConclusionIn the process of colon cancer,there may be some association in epigenetics,and the downregulation of histone acetylation may be another pathway that causes the development of colon cancer.