真菌NBERC＿170302中次生代谢产物Viridicatumtoxin A的分离纯化及农药活性

Isolation,Purification and Pesticide Activity of the Secondary Metabolite Viridicatumtoxin A from Fungi NBERC＿170302

真菌次生代谢产物一直以来都是药物及其先导化合物的重要来源。在真菌源农药活性天然产物的筛选过程中,得到1株具有杀菌和杀虫活性的真菌NBERC_170302,通过活性跟踪以及菌株大量发酵,经提取和制备高效液相色谱分离纯化得到其中的活性化合物,并通过X-射线单晶衍射鉴定为Viridicatumtoxin A,进一步农药活性测试表明,对多种农业致病真菌具有抑制作用,其中对于小麦赤霉菌、灰霉菌和小麦颖枯病菌具有明显活性,并具有中等杀小菜蛾的活性。

Secondary metabolites from fungal have always been an important source of drugs and leading compounds. In the search for pesticides of natural products from fungus, fungus NBERC_170302 with fungicidal and insecticidal activity was obtained. By active tracking and large-scale fermentation, the active compound was obtained and identified as Viridicatumtoxin A by LC-MS and X-Ray diffraction. Viridicatumtoxin A was found to show fungicidal activities to several agricultural pathogenic bacteria, and insecticidal activity to Plutella xylostella by further bioassay.