摘要
凝集素类受体蛋白激酶(Lectin-like receptor kinase,Lec RK)是一类植物特有并分布广泛的蛋白激酶,但水稻(Oryza sativa L.)中OsLecRK家族的亚细胞定位及其生物学功能尚未清楚。依据NCBI公布的水稻OsLecRK的ORF序列设计引物,从水稻粳稻品种叶片cDNA中扩增得到目的片段,构建OsLecRK-GFP融合瞬时表达载体,转化水稻原生质体对其进行亚细胞定位。激光共聚焦观察结果表明,水稻OsLecRK蛋白主要存在于细胞质,为进一步研究其功能奠定了基础。
Lectin-like receptor kinase（Lec RK） is a family of proteins which is unique and universal in plants. However, subcellular localization and biological function of OsLecRK family in rice（Oryza sativa L.） are largely unknown. OsLecRK gene was amplified from Nipponbare leaves cDNA based on the published ORF sequence on NCBI. Subcellular localization of OsLecRK protein was conducted by construction of transient expression vector OsLecRK-GFP and transformation of rice protoplasts. The confocal microscopy results showed that OsLecRK protein was located mainly in the cytoplasm, and built a good foundation for further research on the function of OsLecRK.
作者
闸雯俊
杨芳
张艳茗
游艾青
ZHA Wen-jun;YANG Fang;ZHANG Yan-ming;YOU Ai-qing(Food Crops Institute,Hubei Academy of Agricultural Seienees/Hubei Key Laboratory of Food Crop Germplasms and Genetic hnprovement,Wuhan 430064,China;National Key Laboratory of Hybrid Rice/College of Life Sciences,Wuhan University,Wuhan 430072,China;Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou 434025,Hubei,China)
出处
《湖北农业科学》
2018年第17期111-113,129,共4页
Hubei Agricultural Sciences
基金
国家青年科学基金项目(31501654)
杂交水稻国家重点实验室开放课题基金(KF201801)
